Dataset Information

Effects of Heat Stress on Liver Function Damage and Mechanisms in Pregnant Sows

ABSTRACT: This study aims to investigate the effects and mechanisms of heat stress (HS) on the physiological functions of the liver in primiparous sows during early pregnancy.In the experimental design, we exposed early pregnant sows to HS (high temperature and humidity environment) and TN (normal environment) conditions. Through blood gas analysis, endocrine index detection, biochemical detection, and histological immunofluorescence analysis, we evaluated the systemic physiological responses and liver damage in sows under HS.Additionally, the study carried out preliminary transcriptomics, proteomics, and metabolomics analyses of the liver to reveal the molecular mechanisms underlying liver dysfunction caused by HS.

INSTRUMENT(S):

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Liver

SUBMITTER: 振浩温

LAB HEAD: wen zhenhao

PROVIDER: PXD059676 | Pride | 2025-07-07

REPOSITORIES: Pride

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Publications

Multi-Omics Analysis of Chronic Heat Stress-Induced Biological Effects, Liver Injury, and Heat Tolerance Mechanisms via Oxidative and Anti-Inflammatory Pathways in Early-Pregnancy Sows.

Chai Jie J Wen Zhenhao Z Chen Li L Pu Qiang Q Luo Taorun T Wu Xiaoqian X Ma Zihan Z Luo Zonggang Z Luo Jia J Wang Jingyong J

Antioxidants (Basel, Switzerland) 20250523 6

The prenatal environment critically influences sow and offspring health, with the liver being highly susceptible to heat stress (HS) and vital for antioxidant defense. However, mechanisms underlying HS impacts on early pregnancy and hepatic adaptation remain unclear. This study applied multi-omics to analyze chronic HS responses in early-pregnancy sows. Results demonstrated that HS reduced blood oxygen (PO<sub>2</sub>) and basophils while elevating red blood cell parameters (RBC, HGB, and HCT). ...[more]

PMID: 40563258

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Project description:The seminal plasma (SP) is the liquid component of semen that facilitates sperm transport through the female genital tract. SP modulates the activity of the ovary, oviductal environment and uterine function during the periovulatory and early pregnancy period. Extracellular vesicles (EVs) secreted in the oviduct (oEVs) and uterus (uEVs) have been shown to influence the expression of endometrial genes that regulate fertilization and early embryo development. In some species, semen is composed of well-separated fractions that vary in concentration of spermatozoa and SP composition and volume. This study aimed to investigate the impact of different accumulative fractions of the porcine ejaculate (F1, composed of the sperm-rich fraction (SRF); F2, composed of F1 plus the intermediate fraction; F3, composed of F2 plus the post-SRF) on oEVs and uEVs protein cargo. Six days after the onset of estrus, we determined the oEVs and uEVs size and protein concentration in pregnant sows by artificial insemination (AI-sows) and in non-inseminated sows as control (C-sows). We also identified the main proteins in oEVs and uEVs, in AI-F1, AI-F2, AI-F3, and C-sows. Our results indicated that although the size of EVs is similar between AI- and C-sows, the protein concentration of both oEVs and uEVs was significantly lower in AI-sows (p < 0.05). Proteomic analysis identified 38 unique proteins in oEVs from AI-sows, mainly involved in protein stabilization, glycolytic and carbohydrate processes. The uEVs from AI-sows showed the presence of 43 unique proteins, including already-known fertility-related proteins (EZR, HSPAA901, PDS). We also demonstrated that the protein composition of oEVs and uEVs differed depending on the seminal fraction(s) inseminated (F1, F2, or F3). In conclusion, we have found a specific protein cargo in uterine and oviductal EVs depending on the type of semen fraction the sow was inseminated with, and these insemination with different seminal fractions results in the oviductal and uterine secretion of specific EVs proteins are closely associated with reproductive processes.

			Action	DRS
	2484986_protein_genomic.fasta	Fasta
	Astral-RHC_H_li1.raw	Raw
	Astral-RHC_H_li2.raw	Raw
	Astral-RHC_H_li3.raw	Raw
	Astral-RHS_H_li1.raw	Raw