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Human glioma cells CELSR2 knock-down LC-MSMS

ABSTRACT: This project examined changes in the protein expression profile of human glioma cells and glioma cells after celsr2 knockdown

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Aimei Liu

LAB HEAD: Aimei Liu

PROVIDER: PXD060044 | Pride | 2025-11-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	V87_1_RB1_1_11180.d.zip	Other
	V87_2_RB1_1_11182.d.zip	Other
	V87_3_RB1_1_11184.d.zip	Other
	V87_4_RB1_1_11173.d.zip	Other
	V87_5_RB1_1_11175.d.zip	Other

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Publications

Atypical cadherin CELSR2 acts as a therapeutic target for glioma through WNT3A/β-catenin signaling.

Liu Aimei A Geng Xin X Li Xinyue X Xi Yue Y Han Qing Q Wang Xiangyu X Shen Yajing Y Zhou Libing L

Cell death & disease 20251103 1

Glioma is the most common primary brain tumors and has a high recurrence and mortality rate after surgery. Most gliomas are of astrocytic origin. We recently demonstrated that Celsr2 is essential for injury-induced responses and functions of astrocytes, while its role in the development and treatment of gliomas remains unexplored. In this study, an increase of CELSR2 expression was identified in patient glioma samples and glioma cell lines, and higher levels of CELSR2 correlate with poorer patie ...[more]

PMID: 41184253

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Identification of Zdhhc20 knock-down cell palmitome

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Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells

Project description:HOX genes encode a family of homeodomain-containing transcription factors involved in the determination of cell fate and identity during embryonic development. They also behave as oncogenes in some malignancies. In this study, we found high expression of the HOXD9 gene transcript in glioma cell lines and human glioma tissues by quantitative real-time PCR. Using immunocytochemistry, we observed HOXD9 protein expression in human brain tumor tissues, including astrocytomas and glioblastomas. To investigate the role of HOXD9 in gliomas, we silenced its expression in the glioma cell line U87 using HOXD9-specific siRNA, and observed decreased cell proliferation, cell cycle arrest, and induction of apoptosis. It was suggested that HOXD9 contributes to both cell proliferation and/or cell survival. The HOXD9 gene was highly expressed in a side population (SP) of SK-MG-1 cells that was previously identified as an enriched-cell fraction of glioma cancer stem-like cells. HOXD9 siRNA treatment of SK-MG-1 SP cells resulted in reduced cell proliferation. Finally, we cultured human glioma cancer stem cells (GCSCs) from patient specimens found with high expression of HOXD9 in GCSCs compared with normal astrocyte cells and neural stem/progenitor cells (NSPCs). Our results suggest that HOXD9 may be a novel marker of GCSCs and cell proliferation and/or survival factor in gliomas and glioma cancer stem-like cells, and a potential therapeutic target. we analyzed the expression and function of HOXD9 in human gliomas and found high expression of HOXD9 in GCSCs. HOXD9 contributes to cell proliferation and/or survival in glioma cells and glioma cancer stem-like cells.

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nuclear proteomics of glioma cells infected with an oncolytic adenovirus

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