Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Magnetic nanoparticles influence the biological function of mesenchymal stem cells

ABSTRACT: This project examined changes in the protein expression profile of mesenchymal stem cells and mesenchymal stem cells that ingested magnetic nanomaterials

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Aimei Liu

LAB HEAD: Aimei Liu

PROVIDER: PXD062622 | Pride | 2025-08-04

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	C1_RB1_1_45661.zip	Other
	C2_RB1_1_45663.d.zip	Other
	C3_RB1_1_45665.d.zip	Other
	T1_RB1_1_45668.d.zip	Other
	T2_RB1_1_45670.d.zip	Other

Items per page:

1 - 5 of 7

Publications

Magnetic nanoparticles influence the biological function of mesenchymal stem cells.

Li Xinyue X Qin Sheng S Liao Xiaoyun X Li Yang Y Liu Aimei A

Scientific reports 20250730 1

Mesenchymal' stem cells (MSC) are widely used for transplantation to treat various diseases due to their strong immune regulatory and tissue repair abilities. Magnetic nanoparticles (MNPs) can be used to track transplanted MSC. However, the potential impact of the MNPs we developed on MSC function remains unclear. In this study, we treated MSC with poly-L-lysine (PLL)-modified MNPs (MSC-MNPs) at a concentration of 0.1 µg/µL to assess their effects on MSC. The results showed that there were no si ...[more]

PMID: 40739120

Similar Datasets

Proteomic dissection of nanoparticle-containing vesicles reveals a major role for a macropinocytic-like uptake mechanism and multivesicular bodies during intracellular nanoparticle trafficking

Project description:Rational design of nanocarriers for drug delivery approaches requires an unbiased knowledge of uptake mechanisms and intracellular trafficking pathways. Here we dissected these processes using a quantitative proteomics approach. We isolated intracellular vesicles containing superparamagnetic iron oxide polystyrene nanoparticles and analyzed their protein composition by label free quantitative mass spectrometry. The proteomic snapshot of organelle marker proteins revealed that an atypical macropinocytic-like mechanism mediated the entry of nanoparticles. We show that the entry mechanism is controlled by actin reorganization, atypical macropinocytic signaling and ADP-ribosylation factor 1. Additionally, our proteomics data demonstrated a central role for multivesicular bodies and multilamellar lysosomes in trafficking and final nanoparticle storage. This was confirmed by confocal microscopy and cryo-TEM measurements. By quantitatively analyzing the protein composition of nanoparticle-containing vesicles, our study clearly defines the routes of nanoparticle entry, intracellular trafficking and the proteomic milieu of a nanoparticle-containing vesicle.

2014-09-25 | PXD001175 | Pride

Amorphous silica nanoparticle exposures can lead to mitochondrial protein changes

Project description:Mitochondrial fractions from J774 mouse macrophage/monocyte cells were exposed to amorphous silica nanoforms and mitochondrial protein profiles were examined to gain information on relative toxicities of these nanomaterials on mitochondrial pathways and identify potency determinants, for risk characterization. The applicability of this approach for screening mitochondrial toxicity of nanomaterials was also verified.

2023-03-22 | PXD027593 | Pride

Effects of seed priming mediated by iron oxide magnetic nanoparticles (MNPs) in Capsicum annuum

Project description:Nanotechnology has the potential to revolutionize agriculture by developing engineered nanomaterials to be used as biostimulants, fertilizers, pesticides or smart sensors. Seed priming may represent an opportunity for nano-enabled plant technology to match economic, agronomic and environmental needs. This study investigates the effects of seed priming mediated by iron oxide magnetic nanoparticles (MNPs) in plants. We performed a multilevel integrated study to understand the basic interactions between MNPs and seeds in pepper (Capsicum annuum). Moreover, phenotypic, physiological and molecular analyses were performed to elucidate the biological impact of MNPs from seed to plant development. Interestingly, our findings show positive effects of MNPs on vegetative growth and a profound impact on pepper gene expression patterns. Indeed, we found 2,204 differentially expressed transcripts in nanoprimed seeds, most of them involved in plant defence mechanisms, potentially establishing a seed memory that might enhance the plant's capacity to counteract diverse forms of stress. In conclusion, this work provides a comprehensive investigation about nanoparticle-seed interactions with interesting implications for agricultural technology.

2023-11-08 | E-MTAB-13087 | biostudies-arrayexpress

Toxicogenomic Responses of Nanotoxicity in Daphnia magna Exposed to Silver Nitrate and Coated Silver Nanoparticles

Project description:Applications for silver nanomaterials in consumer products are rapidly expanding, creating an urgent need for toxicological examination of the exposure potential and ecological effects of silver nanoparticles (AgNPs). The integration of genomic techniques into environmental toxicology has presented new avenues to develop exposure biomarkers and investigate the mode of toxicity of novel chemicals. In the present study we used a 15k oligonucleotide microarray for Daphnia magna, a freshwater crustacean and common indicator species for toxicity, to differentiate between particle specific and ionic silver toxicity and to develop exposure biomarkers for citrate-coated and PVP-coated AgNPs. Gene expression profiles revealed that AgNO3 and AgNPs have distinct expression profiles suggesting different modes of toxicity. However, the gene expression profiles of the different coated AgNPs were similar revealing similarities in the cellular effects of these two particles. Major biological processes disrupted by the AgNPs include protein metabolism and signal transduction. In contrast, AgNO3 caused a downregulation of developmental processes, particularly in sensory development. Metal responsive and DNA damage repair genes were induced by the PVP AgNPs, but not the other treatments. In addition, two specific biomarkers were developed for the environmental detection of PVP AgNPs; although further verification under different environmental conditions is needed. We exposed Daphnia magna to the 1/10 LC50 and LC25 of citrate coated and PVP-coated Ag nanoparticles and Ag+ as AgNO3 for 24-h. For each exposure condition, we performed 6 replicate exposures with 5 individuals in each. All exposures were compared to a unexposed laboratory control.

2012-08-04 | E-GEOD-35150 | biostudies-arrayexpress

Proteomic profiling of human mesenchymal stem cells derived from umbilical cord or adipose tissue

Project description:We performed a proteomic profiling of human mesenchymal stem cells (hMSCs) derived from adipose tissue or umbilical cord.

2018-07-11 | PXD009893 | Pride

Human transcripts up-regulated in the human mesenchymal stem cells migrated within the synovium

Project description:After the injection of human synovial mesenchymal stem cells into the rat (Lewis strain) knee joint, synovium was investigated using species specific microarray analyses of human and rat transcripts to investigate the gene expression change after injection. Human synovial mesenchymal stem cells were injected into the rat anterior cruciate ligament transected knee. After harvesting the synovium and extraction of RNA, species specific microarray analyses were performed uisng Affymetrix microarrays to investigate the gene expression change of human and rat transcripts after injection.

2014-09-22 | E-GEOD-61617 | biostudies-arrayexpress

Expression data from mesenchymal stem cells of ossifying fibroma

Project description:Ossifying fibroma (OF) is a common benign fibro-osseous neoplasm of orofacial bones, showing progressive enlargement of the affected jaw with deficiency in bone formation. Our aim is to discover the molecular difference between mesenchymal stem cells from OF and normal jaw bone to provide basis for development of clinical therapy. Global gene expression comparison and gene ontology analysis found that several singaling pathways are involved in the process, including TGFb signaling and Notch signaling. Inhibition of TGFb signaling in mesenchymal stem cells of OF can increase the osteogenesis and decrease proliferation, shedding light on developing strategies for therapy. RNA from mesenchymal stem cells of OF is compared to that of normal jaw bone.

2013-07-24 | E-GEOD-49165 | biostudies-arrayexpress

Human OEhMSCs on Gelfoam or bioconditioned gelfoam

Project description:Human osteogenically enhanced mesenchymal stem cells were cultured for 5 days on standard gelfoam or gelfoam coated with MSC-derived extracellular matrix To address whether mesenchymal stem cell derived extracellular matrix might stimulate the secretion of osteogenic factors by OEhMSCs, we seeded them onto standard gelfoam or ECM-coated gelfoam in vitro and cultured them under standard conditions. After 5 days, we performed transcriptome micro-array analysis to identify genes that were up-regulated >2-fold.

2014-08-22 | E-GEOD-60616 | biostudies-arrayexpress

miRNA profiling of human umbilical cord derived mesenchymal stem cells in the process of smooth muscle cell differentiation

Project description:Cell samples of undifferentiated human umbilical cord mesenchymal stem cells (1-3) and cells that have been cultured in smooth muscle differentiation medium for 6 hours (4-6) and 24 hours (7-9) were collected and subjected to miRNA array. Exploration of miRNA involved smooth muscle differentiation mechanism would offer potential therapeutic choices for improving performance of vascular grafts engineered with umbilical cord mesenchymal stem cells.

2018-01-04 | E-MTAB-6393 | biostudies-arrayexpress

Systems biology approaches reveal low-dose effects of nanoparticles

Project description:Here, we show that systems biology approaches can uncover mechanisms underlying cellular responses to nanomaterials. Using RNA Seq, we found that cationic nanoparticles are capable of triggering down-regulation of cell cycle-related genes in primary human bronchial epithelial cells at doses that do not elicit acute cytotoxicity. Bioinformatics analyses implicated NF-kappaB as a putative transcriptional regulator and functional assays confirmed that cationic nanoparticles caused NF-kappaB-dependent cell cycle arrest. Our study demonstrates the feasibility of applying systems biology tools to assess cellular responses to nanomaterials, not least at low doses.

2015-01-17 | E-MTAB-2397 | biostudies-arrayexpress