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Proteomic profiling of human mesenchymal stem cells derived from umbilical cord or adipose tissue

ABSTRACT: We performed a proteomic profiling of human mesenchymal stem cells (hMSCs) derived from adipose tissue or umbilical cord.

INSTRUMENT(S): TripleTOF 5600, Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Umbilical Cord, Mesenchymal Cell, Adipose Tissue

SUBMITTER: Miguel Marcilla

LAB HEAD: Fernando Corrales

PROVIDER: PXD009893 | Pride | 2018-07-11

REPOSITORIES: Pride

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Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord.

Clemente Luis Felipe LF Hernáez María Luisa ML Ramos-Fernández Antonio A Ligero Gertrudis G Gil Concha C Corrales Fernando José FJ Marcilla Miguel M

Journal of proteome research 20180706 12

Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labe ...[more]

PMID: 29974749

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