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Mass spectrometry of co-immunoprecipitation using tobacco Maturase K as bait

ABSTRACT: Chloroplast group IIA introns derive from bacterial ribozymes. Their splicing likely requires Maturase K (MatK), which has been largely inaccessible to functional analyses being itself a chloroplast intron-encoded protein. Here we used co-immunoprecipitation coupled with proteomics to identify proteins bound to MatK in tobacco (Nicotiana tabacum) seedlings. We used homoplastomic tobacco lines modified to express NtMatK-HA (line NtMatK C+) or control lines carrying only the selection marker (line NtMatK C-). Proteins co-precipitating with NtMatK-HA were purified via anti-HA beads and identified via shotgun proteomics (n=3 seedling pools). The mass spectrometry data were further processed using Philosopher followed by label-free quantification based on precursor intensities using IonQuant and statistical analysis using Amica.

INSTRUMENT(S):

ORGANISM(S): Nicotiana Tabacum (common Tobacco)

TISSUE(S): Seedling

SUBMITTER: Barbara Pfister

LAB HEAD: Samuel C. Zeeman

PROVIDER: PXD060055 | Pride | 2026-02-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2023-06-19-decoys-uniprot-compressed_true_download_true_format_fasta_query__28_28prote-2023.06.19-10.39.21.40.fasta	Fasta
	20230616_C32024_002_S522420_650_MatK_C_1_C_.raw	Raw
	20230616_C32024_002_S522420_650_MatK_C_1_C__uncalibrated.mzML	Mzml
	20230616_C32024_003_S522422_650_MatK_C_3_C_.raw	Raw
	20230616_C32024_003_S522422_650_MatK_C_3_C__uncalibrated.mzML	Mzml

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Mass spectrometry of co-immunoprecipitation using tobacco Maturase K as bait

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