Dataset Information

Integral membrane proteome of adult mouse brain and other organs

ABSTRACT: LC-MS/MS analysis with label-free quantification was used to determine the expression levels of ion transporters, particularly those involved in Ca2+ homeostasis

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, Heart, Brain, Skeletal Muscle, Intestine, Lung, Liver, Urinary Bladder, Kidney

SUBMITTER: Alexander Haupt

LAB HEAD: Prof. Dr. Bernd Fakler

PROVIDER: PXD060109 | Pride | 2025-08-25

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	wt-mouse-bladder-membrane.dat	Other
	wt-mouse-bladder-membrane.mgf	Mgf
	wt-mouse-bladder-membrane.mzId	Mzid
	wt-mouse-bladder-membrane.raw	Raw
	wt-mouse-cerebellum-membrane.dat	Other

Items per page:

1 - 5 of 52

Publications

Ca2+-pumping by PMCA-neuroplastin complexes operates in the kiloHertz-range.

Constantin Cristina E CE Schmidt Barbara B Schwarz Yvonne Y Harada Harumi H Kollewe Astrid A Müller Catrin S CS Henrich Sebastian S Gaal Botond B Kulik Akos A Bruns Dieter D Schulte Uwe U Rieger Heiko H Fakler Bernd B

Nature communications 20250820 1

Ca2+-ATPases in the plasma membrane extrude Ca2+ ions from the cytosol to the extracellular space thereby terminating Ca2+-signals and controlling Ca2+-homeostasis in any type of cell. Recently, these Ca2+-pumps have been identified as protein complexes of the transporting subunits PMCAs1-4 and the single-span membrane proteins Neuroplastin (NPTN) or Basigin that are obligatory for efficient trafficking of the pump complexes to the surface m ...[more]

PMID: 40835599

Similar Datasets

Project description:Cortical rat neuronal culture, lysis and proteolytic digestion Primary rat cortical neurons were generated from E18 Sprague-Dawley rat embryos with minor modifications (Swanger, Mattheyses et al. 2015, Henderson, Greathouse et al. 2019). Neurons were cultured at a density of 4x105 cells per well in 12-well culture plates (Fisher Scientific, catalog no. 353043). Neurons were cultured in Neurobasal medium (Fisher Scientific, catalog no. 21103-049) supplemented with B27 (Fisher Scientific, catalog no.17504-044). Culture maintenance included a half media change every 2-3 days. At DIV 14, neurons were either treated with 150 ng/mL recombinant NRN1 protein (Abcam, ab69755) or vehicle treated with diH2O for 6 hours. NRN1 concentration was chosen based on published data that identified a plateau in exogenous NRN1 induced effects on transient potassium currents at 150 ng/mL (Yao et al., 2012). After 6 hours neurons were washed 2x with 1 mL 1X phosphate-buffered saline (PBS). To harvest cells, 1 mL 1X PBS + protease inhibitor (Fisher Scientific, catalog no. 78426) was added and cells were centrifuged for 2300rpm for 5 minutes at 4°C. Cell pellets were lysed in 200uL 8M urea buffer and HALT protease and phosphatase inhibitor cocktail (1x final concentration). Lysates were sonicated with a probe sonicator 3 times for 10 s with 10 s intervals at 30% amplitude and cleared of cellular debris by centrifugation in a tabletop centrifuge at 18,000 rcf for 3 minutes at 4° C. Protein concentration was determined by BCA assay and one-dimensional SDS-PAGE gels were run followed by Coomassie blue staining as quality control for protein integrity and equal loading before proceeding to protein digestion. Protein homogenates (50 µg) were diluted with 50 mM NH4HCO3 to a final concentration of less than 2 M urea and then treated with 1 mM dithiothreitol (DTT) at 25°C for 30 minutes, followed by 5 mM iodoacetimide (IAA) at 25°C for 30 minutes in the dark. Protein was digested with 1:100 (w/w) lysyl endopeptidase (Wako) at 25°C for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Pierce) at 25°C. Resulting peptides were desalted with a Sep-Pak C18 column (Waters) and dried under vacuum.

Dataset Information

Integral membrane proteome of adult mouse brain and other organs

Dataset's files

Publications

Ca<sup>2+</sup>-pumping by PMCA-neuroplastin complexes operates in the kiloHertz-range.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Integral membrane proteome of adult mouse brain and other organs

Dataset's files

Publications

Ca&lt;sup&gt;2+&lt;/sup&gt;-pumping by PMCA-neuroplastin complexes operates in the kiloHertz-range.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Ca<sup>2+</sup>-pumping by PMCA-neuroplastin complexes operates in the kiloHertz-range.