Dataset Information

SRNA-mediated crosstalk between cell wall stress and galactose metabolism in Staphylococcus aureus

ABSTRACT: Staphylococcus aureus is an opportunistic pathogen responsible for a wide range of diseases in humans. During infections, this bacterium is exposed to various stresses that target its cell wall, such as oxidative or acid environments as well as various cell wall-acting antimicrobials. S. aureus has effective regulatory systems for responding to environmental stresses enabling the expression of factors necessary for its survival. Bacterial small RNAs (sRNAs) play a crucial role in this adaptation process. In this study, we show that RsaOI, an S. aureus sRNA, accumulates under acid stress conditions. This response is mediated via the two-component system VraSR, which is associated with the cell wall damage response. As a component of the VraSR regulon, RsaOI contributes to the survival of S. aureus under acid stress and affects its susceptibility to glycopeptide antibiotics. To identify RsaOI-dependent changes in the abundance of individual proteins, we analyzed by quantitative proteomics which protein levels were affected by RsaOI expression. Our findings reveal that RsaOI targets lacABCDFEG operon, which encodes components of tagatose pathway, a unique mechanism responsible for galactose metabolism in S. aureus. By antisense base pairing near the ribosome-binding site of lacD, RsaOI inhibits the expression of this gene, encoding tagatose-6-phosphate aldolase. This regulation disrupts the tagatose pathway, impairing galactose utilization in S. aureus. Furthermore, we demonstrated that disruption of the lac operon repression leads to significant alterations in the cell morphology and cell envelope structure. These findings highlight the role of RsaOI in the mediation between cell wall stress responses and specific metabolic pathway.

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ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Julie Hardouin

LAB HEAD: Julie Hardouin

PROVIDER: PXD060402 | Pride | 2025-07-28

REPOSITORIES: Pride

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sRNA-mediated crosstalk between cell wall stress and galactose metabolism in Staphylococcus aureus.

Germain Maëliss M Robin Hugo H Le Huyen Kim Boi KB Massier Sébastien S Nalpas Nicolas N Hardouin Julie J Bouloc Philippe P Rouillon Astrid A Chabelskaya Svetlana S

Nucleic acids research 20250701 13

Staphylococcus aureus is an opportunistic pathogen responsible for a wide range of diseases in humans. During infections, this bacterium is exposed to various stresses that target its cell wall, such as oxidative or acid environments as well as various cell wall-acting antimicrobials. Staphylococcus aureus has effective regulatory systems for responding to environmental stresses, enabling the expression of factors necessary for its survival. Bacterial small RNAs (sRNAs) play a crucial role in th ...[more]

PMID: 40671522

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