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N-terminomics on WT and CASP2 KO mouse hearts

ABSTRACT: To identify putative substrates processed by Caspase-2 in response to PIDDosome activation in cardiomyocytes, we performed N-terminomics by TMT10-TAILS on P7 hearts isolated from XMLC2-Casp2fl/fl and XMLC2+Casp2fl/fl mice.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Piero Giansanti

LAB HEAD: Piero Giansanti

PROVIDER: PXD060421 | Pride | 2026-01-15

REPOSITORIES: Pride

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			Action	DRS
	Ecl1_0474_R0098-02_S008061_L_H01_TMT10_01.raw	Raw
	Ecl1_0474_R0098-02_S008061_L_H01_TMT10_02.raw	Raw
	Ecl1_0474_R0098-02_S008061_L_H02_TMT10_01.raw	Raw
	Ecl1_0474_R0098-02_S008061_L_H02_TMT10_02.raw	Raw
	Ecl1_0474_R0098-02_S008061_L_H03_TMT10_01.raw	Raw

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1 - 5 of 13

Publications

The PIDDosome controls cardiomyocyte polyploidization during postnatal heart development.

Leone M M Kinz N N Eichin F F Obwegs D D Sladky V C VC Braun V Z VZ Hirschberger R R Rizzotto D D Englmaier L L Manzl C C Moos K K Mergner J J Giansanti P P Martinez-Garcia N N Marques M M MM Jacotot E D ED Eblahed L L Yousif R R Wright M K MK Dawood D D Maupome L S LS Savko C C Boerries M M Sussman M A MA Villunger A A

Cell death and differentiation 20260112

The adult mammalian heart is characterized by post-mitotic polyploid cardiomyocytes (CMs). Understanding how CMs regulate cell cycle exit and polyploidy can help developing new heart regenerative therapies. Here, we uncover that the PIDDosome, a multi-protein complex activating the endopeptidase Caspase-2, helps to implement a CM-specific differentiation program that limits ploidy during postnatal heart development. DNA content analyses show that cell-autonomous PIDDosome loss causes an increase ...[more]

PMID: 41526722

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2025-06-06 | PXD062840 | Pride

N-terminomics profiling of host proteins targeted by excretory-secretory proteases of the nematode Angiostrongylus vasorum identifies points of interaction with canine coagulation and complement cascade

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