Proteomics

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Top-down proteomics study of aging and sexual dimorphism of zebrafish brains

ABSTRACT: Here, we present the first example of studying the aging and sexual dimorphism of brains using the zebrafish (Danio rerio) model organism by mass spectrometry (MS)-based quantitative top-down proteomics (TDP). We measured the proteoforms in male and female zebrafish brains across three ages (6, 16, and 24 months) using capillary zone electrophoresis-tandem MS (CZE-MS/MS). Our research indicates that male and female zebrafish exhibit significant differences in the abundance of important proteoforms. Significant age-related changes were observed in proteoforms associated with critical neurological processes, including neurofilament assembly, dopaminergic neuron differentiation, and synaptic vesicle priming.

INSTRUMENT(S):

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Brain

SUBMITTER: Liangliang Sun  

LAB HEAD: Liangliang Sun

PROVIDER: PXD060426 | Pride | 2026-01-12

REPOSITORIES: Pride

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Female_16_1.raw Raw
Female_16_1_ms2_toppic_proteoform_single.tsv Tabular
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Female_16_2_ms2_toppic_proteoform_single.tsv Tabular
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Top-Down Proteomics Study of Aging and Sexual Dimorphism of Zebrafish Brains.

Askarani Mehrdad Falamarzi MF   Poulos William W   Dashtaki Maryam Rahimzadeh MR   Sadeghi Seyed Amirhossein SA   Cibelli Jose B JB   Fang Fei F   Sun Liangliang L  

Journal of mass spectrometry : JMS 20260101 1

Aging significantly impacts brain function and increases susceptibility to neurodegenerative diseases, with notable sex dimorphism observed in aging-related dementia, such as Alzheimer's disease. To better understand the molecular mechanisms of aging and dementia, it is essential to globally and accurately characterize biomolecules (e.g., proteins) in the brain as a function of age and sex. Here, we present one of the first studies of aging and sexual dimorphism in brains using the zebrafish (Da  ...[more]

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