Top-down proteomics study of aging and sexual dimorphism of zebrafish brains

ABSTRACT: Here, we present the first example of studying the aging and sexual dimorphism of brains using the zebrafish (Danio rerio) model organism by mass spectrometry (MS)-based quantitative top-down proteomics (TDP). We measured the proteoforms in male and female zebrafish brains across three ages (6, 16, and 24 months) using capillary zone electrophoresis-tandem MS (CZE-MS/MS). Our research indicates that male and female zebrafish exhibit significant differences in the abundance of important proteoforms. Significant age-related changes were observed in proteoforms associated with critical neurological processes, including neurofilament assembly, dopaminergic neuron differentiation, and synaptic vesicle priming.

INSTRUMENT(S):

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Brain

SUBMITTER: Liangliang Sun  

LAB HEAD: Liangliang Sun

PROVIDER: PXD060426 | Pride | 2026-01-12

REPOSITORIES: Pride