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Proteomic Analysis of Huh7, HIEC6, and NCI-H295R cells using High-affinity anti-Golm1 mAb by IP-MS.

ABSTRACT: we conducted IP-MS strategy to screen its interacting proteins. High-affinity anti-Golm1 mAb and IgG were used in multiple cell lines including Huh7, HIEC6, and NCI-H295R cells. Proteins with more than two peptide segments enriched or exclusively within the Golm1 group were identified as putative interacting partners of Golm1.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Ruzhou Zhao

LAB HEAD: Congwen Wei

PROVIDER: PXD061115 | Pride | 2026-03-23

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	H295R.zip	Other
	HIEC.zip	Other
	Huh7.zip	Other

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Publications

The GOLM1-ACLY pathway regulates macrophage-secreted EFEMP1 via H3K27ac modifications to drive tumor progression.

Zhen Lan L Min Min M Mo Xinglin X Zhao Guilin G Li Huilong H Liu Muyi M Wan Luming L Yang Xiaopan X Huang Linfei L Zhang Yanhong Y Gao Qi Q Zhong Hui H Qiang Guangliang G Wu Feixiang F Xie Qiaosheng Q Wei Congwen C Zhao Ruzhou R

Journal of advanced research 20250704

<h4>Background</h4>Macrophages play crucial regulatory roles within the tumor immune microenvironment (TIME) and significantly influence tumor progression. GOLM1 has been identified as being closely associated with a variety of cancers. Nevertheless, the role of GOLM1 in macrophages on tumor progression remains unknown.<h4>Objectives</h4>This study aims to investigate the mechanism by which the GOLM1-ACLY axis in macrophages regulates tumor progression.<h4>Methods</h4>The orthotopic hepatocellul ...[more]

PMID: 40619122

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