Dataset Information

Bulk proteomics of naive human dorsal root ganglion

ABSTRACT: Study Purpose: This study set out to a) provide a quantitative proteomic reference for the human DRG and nerve root and b) explore sexual dimorphism in these tissues. Data Collection: DIA-PASEF proteomics was performed on hDRG tissue from donors. Primary Conclusion: Multi-omic integration with human DRG proteomics highlights TNFa signaling as a relevant sexually dimorphic pathway in the periphery.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Peripheral Nerve, Peripheral Ganglion

SUBMITTER: Allison Barry

LAB HEAD: Manuela Schmidt

PROVIDER: PXD061129 | Pride | 2025-09-22

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 4 of 4

Publications

Multi-omic integration with human dorsal root ganglia proteomics highlights TNFα signalling as a relevant sexually dimorphic pathway.

Barry Allison M AM Sondermann Julia R JR Lesnak Joseph B JB Xian Feng F Franco-Enzástiga Úrzula Ú O'Brien Jayden A JA Gomez-Varela David D Schackmuth Morgan K MK Shiers Stephanie S Price Theodore J TJ Schmidt Manuela M

Pain 20250520 10

<h4>Abstract</h4>The peripheral nervous system (PNS) plays a critical role in pathological conditions, including chronic pain disorders, that manifest differently in men and women. To investigate this sexual dimorphism at the molecular level, we integrated quantitative proteomic profiling of human dorsal root ganglia (hDRG) and peripheral nerve tissue into the expanding omics framework of the PNS. Using data-independent acquisition (DIA) mass spectrometry, we characterized a comprehensive proteo ...[more]

PMID: 40388638

Publication: 1/2

Similar Datasets

Project description:Vagal afferent neurons are thought to convey primarily physiological information, whereas spinal afferents transmit noxious signals from the viscera to the central nervous system. In order to elucidate molecular identities for these different properties, we compared gene expression profiles of neurons located in nodose ganglia (NG) and dorsal root ganglia (DRG) in mice. Intraperitoneal administration of Alexa Fluor-488 conjugated Cholera toxin B allowed identification of neurons projecting to the viscera. Fluorescent neurons in DRG (from T10 to T13) and NG were isolated using laser capture microdissection. Gene expression profiles of visceral afferent neurons, obtained by microarray hybridization, were analysed using multivariate spectral map analysis, SAM algorithm (Significance Analysis of Microarray data) and fold-difference filtering. A total of 1996 genes were found to be differentially expressed in DRG versus NG, including 41 G-protein coupled receptors and 60 ion channels. Expression profiles obtained on laser-captured neurons were contrasted to those obtained on whole ganglia demonstrating striking differences and the need for microdissection when studying visceral sensory neurons because of dilution of the signal by somatic sensory neurons. Furthermore, a detailed catalogue of all adrenergic and cholinergic, GABA, glutamate, serotonin and dopamine receptors, voltage-gated potassium, sodium and calcium channels and transient receptor potential cation channels present in visceral afferents is provided. Our genome-wide expression profiling data provide novel insight into molecular signatures that underlie both functional differences and similarities between NG and DRG visceral sensory neurons. Moreover, these findings will offer novel insight into mode of action of pharmacologic agents modulating visceral sensation. Experiment Overall Design: Three separate experiments were performed. First, 5 whole dorsal root ganglia were compared to 7 whole nodose ganglia. Second, Laser captured visceral neurons derived from 5 dorsal root ganglia and 5 nodose ganglia were compared on MG-U74Av2. Third, Laser captured visceral neurons derived from 9 dorsal root ganglia and 11 nodose ganglia were compared on Mouse430_2.

			Action	DRS
	ST11-ExpressionMatrix.csv	Csv
	samples.xlsx	Xlsx
	subjects.xlsx	Xlsx
	timsTOF.zip	Other