Dataset Information

Conformational landscapes of rigid and flexible molecules explored with variable temperature ion mobility-mass spectrometry

ABSTRACT: Variable temperature ion mobility-mass spectrometry (VT-IM-MS) can measure the effect of temperature on conformational landscapes. To delineate collision effects from structural change we report measurements using molecules with different degrees of rigidity namely: poly (L-lysine) (PLL) dendrimer, ubiquitin, β-casein and α-synuclein from 190-350 K. The CCS of PLL dendrimer varies with temperature consistent with collision theory, by contrast, the structure of each protein alters with notable restructuring at 350 K and 250 K, following predicted in vitro stability curves. At 210 K and 190 K we kinetically trap unfolding intermediates. For alpha-synuclein, the 13+ ions present two distinct conformers and VT-IM-MS measurements allow us to calculate the transition rate and activation energies of their conversion. These data exemplify the capacity of VT-IM-MS to provide insights to thermodynamics of conformational restructuring.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jason M D Kalapothakis

LAB HEAD: Perdita E. Barran

PROVIDER: PXD061503 | Pride | 2026-02-23

REPOSITORIES: Pride

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Conformational landscapes of rigid and flexible molecules explored with variable temperature ion mobility-mass spectrometry.

Wang Xudong X Norgate Emma E Dai Junxiao J Benoit Florian F Bristow Tony T England Richard M RM Kalapothakis Jason M D JMD Barran Perdita E PE

Nature communications 20250506 1

Understanding the effect of temperature to the structural integrity of proteins is relevant to diverse areas such as biotechnology and climate change. Variable temperature ion mobility-mass spectrometry (VT-IM-MS) can measure the effect of temperature on conformational landscapes. To delineate collision effects from structural change we report measurements using molecules with different degrees of rigidity namely: poly (L-lysine) (PLL) dendrimer, ubiquitin, β-casein and α-synuclein from 190-350 ...[more]

PMID: 40324998

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