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Dynamic Organellar Mapping in yeast exposed to ER stress

ABSTRACT: Sophisticated techniques are available for systematic studies of yeast cell biology. However, it remains challenging to investigate protein subcellular localization changes on a proteome-wide scale. Here, we apply Dynamic Organellar Maps (DOMs) by label-free mass spectrometry to detect localization changes of native, untagged proteins during endoplasmic reticulum (ER) stress. We find that hundreds of proteins shift between cellular compartments. For example, we show that numerous secretory pathway proteins accumulate in the ER, thus defining the extent and selectivity of ER retention of misfolded proteins. Furthermore, we identify candidate cargo proteins of the ER reflux pathway, determine constituents of reticulon clusters that segregate from the remainder of the ER and provide evidence for altered nuclear pore complex composition and nuclear import. These findings uncover protein relocalization as a major aspect of cellular reorganization during ER stress and establish DOMs as a powerful discovery tool in yeast. This dataset comprises the comparative experiment comparing untreated cells, with ones treated with either DTT or tunicamycin. The data analysis reveals a multitude of changes in a classical volcano analysis as well as in the multivariate outlier analysis of subcellular localization changes.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) Saccharomyces Cerevisiae

SUBMITTER: Julia Schessner

LAB HEAD: Georg H H Borner

PROVIDER: PXD061764 | Pride | 2025-12-03

REPOSITORIES: Pride

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			Action	DRS
	20230117_EXPL1_GeBo_S436_BL115_yeastmaps_SA_LFQ_DDA_Control_map1_12k_SS.raw	Raw
	20230117_EXPL1_GeBo_S436_BL115_yeastmaps_SA_LFQ_DDA_Control_map1_1k_SS.raw	Raw
	20230117_EXPL1_GeBo_S436_BL115_yeastmaps_SA_LFQ_DDA_Control_map1_24k_SS.raw	Raw
	20230117_EXPL1_GeBo_S436_BL115_yeastmaps_SA_LFQ_DDA_Control_map1_3k_SS.raw	Raw
	20230117_EXPL1_GeBo_S436_BL115_yeastmaps_SA_LFQ_DDA_Control_map1_6k_SS.raw	Raw

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Dynamic Organellar Mapping in yeast exposed to ER stress

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