Dataset Information

Phosphate refeeding in Arabidopsis thaliana seedlings exerts differential impacts on the shoot and root proteome.

ABSTRACT: Phosphorus (P) is an essential plant macronutrient required for fundamental biochemical, metabolic, and physiological processes. In its inorganic and chemically available form of orthophosphate (H2PO4-; Pi), plant root epidermal cells are able to directly assimilate this mineral from the soil via plasmalemma Pi transporters (Dissanayaka et al., 2021). The role of Pi in plants is multifaceted; it serves as a central structural component of important biomolecules, including nucleic acids, sugar phosphates, and phospholipids, and is also required for important regulatory processes such as photosynthesis (i.e. triose-P exchange) and respiration. Additionally, Pi allosterically regulates several enzymes of central plant metabolism via covalent attachment to a specific amino acid residue (i.e., phosphorylation). Despite its significance, most soil-Pi levels are extremely suboptimal for sustaining crop growth due its precipitation and subsequent formation of metal cation-Pi complexes (Chen & Liao, 2016). Furthermore, a significant amount of soil-Pi is trapped in organic substrates (e.g. decaying biomatter) which must be mineralized prior to plant uptake (Raghothama, 1999). Typical concentrations of readily available soil-Pi (0.1-10 μM) are thousands of orders of magnitude lower than those found in nutrient-sufficient plant tissue, and as a result, excessive quantities of unsustainable Pi fertilizers sourced from finite ‘rock-Pi’ reserves are applied to crops (Hinsinger, 2001; Raghothama, 1999; Blackwell et al., 2019). In addition to the rapidly depleting levels of global rock-Pi reserves, excess Pi runoff from these fertilizers can leach into aquatic ecosystems causing eutrophication and harmful algal blooms (Lambers & Plaxton, 2015). Given the inefficiency of Pi fertilizers and scarcity of Pi reserves, agricultural solutions are urgently needed to reduce our over-reliance on exogenous Pi application. By studying the adaptations of -Pi plants, we may uncover potential biological targets for engineering Pi-efficient crop varieties.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Root, Shoot

SUBMITTER: Richard Uhrig

LAB HEAD: R. Glen Uhrig

PROVIDER: PXD062427 | Pride | 2026-02-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20220823_Milli_Plaxton_TP_01_48h_NoPi_Shoot_3.raw	Raw
	20220823_Milli_Plaxton_TP_02_48h_PiRS_Shoot_4.raw	Raw
	20220823_Milli_Plaxton_TP_03_48h_PiRS_Root_2.raw	Raw
	20220823_Milli_Plaxton_TP_04_48h_NoPi_Shoot_4.raw	Raw
	20220823_Milli_Plaxton_TP_05_1h_NoPi_Shoot_1.raw	Raw

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Publications

Phosphate Resupply Differentially Impacts the Shoot and Root Proteomes of Arabidopsis thaliana Seedlings.

Smith Milena A MA Grubb Lauren E LE Benidickson Kirsten H KH Mehta Devang D Plaxton William C WC Uhrig R Glen RG

Plant, cell & environment 20251210 3

Phosphate (Pi) is an essential macronutrient for plant development that is often limited in soil. Plants have evolved dynamic biochemical, physiological and morphological adaptations to cope with Pi deficiency, known as the Pi starvation response (PSR). While many components of the PSR have been well-characterised, less is known about how metabolic homoeostasis is re-established upon Pi resupply, particularly tissue- and time-specific adaptations. Here, we applied label-free quantitative proteom ...[more]

PMID: 41368764

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