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DIA-MS workflows for cyanobacterial proteomics

ABSTRACT: In this project we introduce new workflows for the in-depth description of the cyanobacterial proteome. The mainstay features of these workflows are the improvement of sample cleanup and digestion through the use of single-pot solid phase-enhanced sample preparation (SP3), the introduction of a recently developed trifluoroaceticacid lysis strategy, and the application of library-free data independent acquisition.

INSTRUMENT(S):

ORGANISM(S): Synechococcus Elongatus (strain Pcc 7942) (anacystis Nidulans R2)

SUBMITTER: David A. Russo

LAB HEAD: David A. Russo

PROVIDER: PXD062851 | Pride | 2025-09-15

REPOSITORIES: Pride

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			Action	DRS
	199_219_DIA_Spectronaut.zip	Other
	20241216_nElute_tims1_FSc_Russo_15cm_30min_199_Slot1-01_1_9321.d.zip	Other
	20241216_nElute_tims1_FSc_Russo_15cm_30min_200_Slot1-02_1_9323.d.zip	Other
	20241216_nElute_tims1_FSc_Russo_15cm_30min_201_Slot1-03_1_9324.d.zip	Other
	20241216_nElute_tims1_FSc_Russo_15cm_30min_202_Slot1-04_1_9325.d.zip	Other

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Publications

Library-free data-independent acquisition mass spectrometry enables comprehensive coverage of the cyanobacterial proteome.

Russo David A DA Schneidmadel Felix R FR Zedler Julie A Z JAZ

Plant physiology 20250901 1

Cyanobacteria have played a leading role in elucidating the fundamental mechanisms behind oxygenic photosynthesis, carbon fixation, the circadian clock, and phototaxis. Such molecular processes rely on proteins at their core. Thus, proteomics has become an indispensable tool in building our understanding of these processes. Amongst the proteomic approaches used, "shotgun proteomics", where complex protein mixtures are enzymatically digested into peptides and analyzed by liquid chromatography-mas ...[more]

PMID: 40796368

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