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Whole Cell and Ubiquitinated Proteome of the Endoplasmic Reticulum (ER) Stress Response to Mild Hypothermic Culture Conditions in Chinese Hamsters Ovary Cells

ABSTRACT: This study investigates the impact of mild hypothermic conditions (31°C) on the whole cell proteome and ubiquitinated proteome of Chinese hamster ovary (CHO) cells, with a specific focus on endoplasmic reticulum (ER) proteins and ER stress. Using high-resolution mass spectrometry, we conducted a comprehensive proteomic and ubiquitinated proteomic analysis to quantify changes in protein abundance and ubiquitinated peptides under mild hypothermia.

INSTRUMENT(S):

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Cell Culture

SUBMITTER: David Ryan

LAB HEAD: Paula Meleady

PROVIDER: PXD063085 | Pride | 2025-12-22

REPOSITORIES: Pride

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			Action	DRS
	CHOK1_D4_31_A.fasta	Fasta
	CHOK1_D4_31_A.mzML	Mzml
	CHOK1_D4_31_A.mzid	Mzid
	CHOK1_D4_31_A.raw	Raw
	CHOK1_D4_31_A_.fasta	Fasta

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Publications

Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions.

Ryan David D Sideri Christiana-Kondylo CK Henry Michael M Karuppuchamy Selvaprakash S Efeoglu Esen E Meleady Paula P

Biotechnology and bioengineering 20251028 1

Chinese hamster ovary (CHO) cells, widely utilised in biopharmaceutical production, experience various stressors during cell culture that can affect protein expression and folding, particularly within the endoplasmic reticulum (ER). Mild hypothermia is widely employed in CHO cell bioproduction to improve recombinant protein yield and quality; however, its impact on ER-associated pathways, particularly those governing protein folding and stress responses, remains insufficiently characterised. Mas ...[more]

PMID: 41146587

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