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7-Octenoic Acid Exerts Anti-Fibrotic Effects on TGF-β1-Activated Hepatic Stellate Cells: Insights from Proteomic Analysis

ABSTRACT: Liver fibrosis represents the liver’s adaptive response to sustained inflammation triggered by diverse insults such as dietary factors and infectious agents. Although early-stage fibrosis can be reversible, unchecked progression often leads to cirrhosis and hepatocellular carcinoma. Therapeutic options remain scarce, highlighting the urgent need for novel anti‑fibrotic compounds. In this study, we evaluated the in vitro anti‑fibrotic potential of 7‑octenoic acid (7‑OCT), a bioactive constituent identified from Moringa oleifera Lam. Human hepatic stellate LX‑2 cells were stimulated with TGF‑β1 (10 ng/mL) in the presence or absence of 7‑OCT. Fibrotic marker expression was quantified at both the mRNA and protein levels. Comprehensive proteomic profiling and subsequent in silico docking analyses were performed to identify putative molecular targets and clarify mechanisms of action. Our results demonstrate that 7‑OCT significantly suppresses the expression of α‑smooth muscle actin (α‑SMA), collagen type I, and matrix metalloproteinase‑9 (MMP‑9) by downregulating TGF‑β receptor 1 (TGFBR1) expression and inhibiting mothers against decapentaplegic homolog 3 (SMAD3) phosphorylation. Proteomic data further implicate focal adhesion pathway proteins in mediating these effects. Collectively, these preliminary findings suggest that 7‑OCT is a promising lead for the development of anti‑fibrotic therapies.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver, Myofibroblast Cell

SUBMITTER: Watunyoo Buakaew

LAB HEAD: Kanchana Usuwanthim

PROVIDER: PXD063183 | Pride | 2025-11-10

REPOSITORIES: Pride

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			Action	DRS
	7-OCT_01.raw	Raw
	7-OCT_02.raw	Raw
	7-OCT_03.raw	Raw
	Protein_list_7-OCT_vs_TGF-beta.xlsx	Xlsx
	TGF_01.raw	Raw

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