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HCR-Proxy analysis of site-specific pre-rRNA proximal proteomes.

ABSTRACT: We developed a novel method termed HCR-Proxy, a proximity labeling strategy that couples Hybridization Chain Reaction (HCR)-mediated signal amplification with in situ free radical-based biotinylation (Proxy-based labelling), allowing proteomic mapping of RNA-associated proteins with subcompartmental spatial resolution. To benchmark the method, we applied HCR-Proxy to specific sequences of nascent pre-ribosomal RNA (pre-rRNA), enabling the dissection of distinct proteomic landscapes across nucleolar subcompartments associated with distinct pre-rRNA maturation and processing steps.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Faraz Mardakheh

LAB HEAD: Faraz Mardakheh

PROVIDER: PXD064165 | Pride | 2026-02-03

REPOSITORIES: Pride

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			Action	DRS
	20240216_MadForAnja_IP_OnBeadDigest_45S_Rep1.raw	Raw
	20240216_MadForAnja_IP_OnBeadDigest_45S_Rep2.raw	Raw
	20240216_MadForAnja_IP_OnBeadDigest_45S_Rep3.raw	Raw
	20240216_MadForAnja_IP_OnBeadDigest_45S_Rep4.raw	Raw
	20240609_MadforAnja_RNAproxLabel_45S_Rep1_1.raw	Raw

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