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Host Proteins Interacting with Wild-Type and Signal Peptide-Cleaved GP64 of Bombyx mori Nucleopolyhedrovirus (BmNPV)

ABSTRACT: Bombyx mori nucleopolyhedrovirus (BmNPV) relies on the envelope glycoprotein GP64 to mediate host cell entry. Intriguingly, both the wild-type GP64 retaining its signal peptide (SP) and the SP-cleaved variant (SP∆nGP64) can support viral invasion, yet their mechanisms may differ. To identify potential GP64 receptors and dissect host interaction profiles, we performed comparative co-immunoprecipitation using purified GP64 and SP∆nGP64, followed by data-independent acquisition (DIA)-based proteomic analysis. A total of 6,064 and 5,247 host proteins were identified, respectively. Enrichment analyses revealed shared involvement in membrane trafficking, lipid metabolism, and endocytosis—pathways essential for viral entry.

INSTRUMENT(S):

ORGANISM(S): Bombyx Mori (silk Moth)

TISSUE(S): Ovary, Cell Culture

SUBMITTER: Kai Chen

LAB HEAD: Jinshan Huang

PROVIDER: PXD065051 | Pride | 2025-08-31

REPOSITORIES: Pride

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