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Proteomic profiles of brains of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) at different stages of gonadal development

ABSTRACT: For the first time, the proteomic profiles of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) at two maturation stages were described and discussed. Maturation and reproduction are critical stages in the fish's life cycle, but the biochemical and morphophysiological processes underlying these stages are not well understood. The reactions that occur in the fish brain during maturation, regulating the reproductive process and accompanying metabolic changes, are of particular interest. O. mykiss is an economically significant commercial species of salmonid fish that is a model organism for biological research. We analyzed the proteomic profiles of rainbow trout brains at different stages of gonadal development, including stages 2-3 (developing) and stage 5 (spawning). It was revealed that the proteomic profiles brains differed in terms of proteins involved in lipid metabolism, immune responses, stress response, cell structure, and oocyte development. These findings provide valuable insights into the complex processes that occur during fish maturation and can contribute to our understanding of reproductive biology. The biochemical differences in the brain at different stages of development are shown, as well as the fact that the by-products of commercial fishing, in this case, heads and brains, can be used to extract antimicrobial proteins and peptides.

INSTRUMENT(S):

ORGANISM(S): Oncorhynchus Mykiss (rainbow Trout) (salmo Gairdneri)

TISSUE(S): Brain

SUBMITTER: Albina Tsekova

LAB HEAD: Svetlana A. Murzina

PROVIDER: PXD065505 | Pride | 2025-10-29

REPOSITORIES: Pride

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			Action	DRS
	11_mature5_250323SKV_01.mgf	Mgf
	11_mature5_250323SKV_01.mzXML	Mzxml
	11_mature5_250323SKV_01_20230325141129.mgf	Mgf
	11_mature5_250323SKV_01_20230325141129.mzXML	Mzxml
	11_mature5_250323SKV_01_20230325154744.mgf	Mgf

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Project description:A shotgun metagenome microarray was created and used to investigate gene transcription during vinyl chloride (VC) dechlorination by a microbial enrichment culture called KB1. The array was constructed by spotting genomic fragments amplified from short-insert libraries of KB1 metagenomic DNA. Subsequently, the microarrays were interrogated with RNA extracted from KB1 during VC dechlorination (VC+methanol), and in the absence of VC (methanol-only). The most differentially expressed spots, and spots with the highest intensities, were then chosen to be sequenced. Sequencing revealed that Dehalococcoides (Dhc) genes involved in transcription, translation and energy generation were up-regulated during VC degradation. Furthermore, the results indicated that the reductive dehalogenase homologous (RDH) gene KB1rdhA14 is the only RDH gene up-regulated upon VC degradation, and that multiple RDH genes were more highly transcribed in the absence of VC. Numerous hypothetical genes from Dehalococcoides were also more highly transcribed in methanol only treatments and indicate that many uncharacterized proteins are involved in cell maintenance in the absence of chlorinated substrates. Spots with genes from Spirochaetes, Chloroflexi, Geobacter, Methanogens and phage organisms were differentially expressed and sequencing provided information from these uncultivated organisms that can be used to design primers for more targeted studies. This array format is powerful, as it does not require a priori sequence knowledge. This study provides the first report of such arrays being used to investigate transcription in a mixed community, and shows that this array format can be used to screen metagenomic libraries for functionally important genes. 2 Biological replicate experimens conducted 1 month apart. In the first there were 2 dye-swapped duplicates (total 4) of VC+MeOH versus MeOH only. In the second experiment there was one set of dye swapped arrays. Thus 6 arrays were performed including biological replicates, dye swapped replicates and technical duplicates.

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Proteomic profiles of brains of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) at different stages of gonadal development

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