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Sulfoxide-diazirine (SODA) as a cleavable photoaffinity group to enable identification of photolabeled modification sites via LC-MS3

ABSTRACT: Photoaffinity labeling is a common approach in chemical proteomics for identification of small molecule probe tar-gets. However, the modification site on the target protein remains difficult to identify, because of the probe modifica-tion and potential fragmentation during tandem mass spectrometry. We here introduce MS-cleavable photoaffinity groups for a better identification of the modification site. These are based on diazirine photoreactive groups and sul-foxide as MS-labile linker. We have applied these in photoaffinity probes based on the peptide-like aspartic protease inhibitor pepstatin A, and we demonstrate that we can identify and map binding hotspots on structural models of a target protease.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: Steven Verhelst

LAB HEAD: Steven Verhelst

PROVIDER: PXD065837 | Pride | 2025-08-25

REPOSITORIES: Pride

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			Action	DRS
	Detailed-sample-description-final.csv	Csv
	Eclipse_00128-49_1128-8_QuanEnergy_MS3_20ppm_0.5_TD_ptmrs_25Th_Chymo_PeptideGroups.txt	Txt
	Eclipse_00128.raw	Raw
	Eclipse_00129.raw	Raw
	Eclipse_00130.raw	Raw

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