Proteome characterization of single gene deletion mutants lacking zinc finger µ-proteins in Haloferax volcanii

ABSTRACT: µ-proteins (≤ 70 amino acids) have important and often essential roles in all kingdoms of life, including cell motility, regulation of membrane transport and as transcription factors. In the halophilic archaeon and model system Haloferax volcanii a significant number of µ-proteins were predicted to be zinc finger proteins. Here we used mass spectrometry-based proteomics to systematically investigate the impact of single gene deletions of 19, previously uncharacterized, zinc finger µ-proteins on H. volcanii grown in glycerol media. We employed a state-of-the-art dia-PASEF acquisition strategy, detecting over 3400 proteins across the 19 deletion strains and the wild-type. The comprehensive proteome coverage enabled a systematic analysis of proteome remodeling. We found that in 11 out of the 19 mutants the proteome remodeling involved proteins annotated to play a role in cell motility, matching swarming and cell growth phenotypes we observed for these strains. Taken together, our data provide the most comprehensive proteome coverage of H. volcanii to date, and the effect of 19 different zinc-finger µ-proteins deletion strains on the proteome of this organism. The combined data provide a valuable resource for future research in the field.

INSTRUMENT(S):

ORGANISM(S): Haloferax Volcanii (halobacterium Volcanii)

SUBMITTER: Julian Langer  

LAB HEAD: Julian Langer

PROVIDER: PXD066008 | Pride | 2026-02-26

REPOSITORIES: Pride