Dataset Information

Comparative Analysis of the Fecal Proteome in Two Canine Breeds: Dalmatians and Weimaraners

ABSTRACT: The analysis of proteins in stool samples can significantly enhance the study of mamma-lian physiology and disease. In this study, we investigated the fecal proteome of clinically healthy dogs (n = 26) by a label-free proteomics approach to evaluate the impact of breed differences. The dogs were split into two groups (n = 13 each) based on their breed, specif-ically Weimaraner and Dalmatian, the former known for their possible susceptibility to gastrointestinal disease. Quantitative and qualitative differences between the two experi-mental groups were identified based on analyses performed on pooled biological sam-ples,. The overall fecal proteome profile comprised 58 proteins, of which 37 were in com-mon, while the comparative proteomics analysis detected 15 proteins having different abundance. Notably, the fecal proteome of Weimaraners showed an over-representation of proteins such as pantetheinase, which promotes inflammatory reactions; ferritin heavy chain and hemoglobin, possibly associated with gut ulceration and/or rectal bleeding typical of IBD; and anionic trypsin, implicated in inflammatory bowel disease. Notably, in Dalmatians, despite the absence of specific predispositions, some proteins associated with chronic enteropathy (e.g., carboxypeptidase B and serine protease 1) were also over-represented. Additionally, some proteins linked to breed variation included enzymes associated with “protein digestion and absorption” and “glycolysis and gluconeogene-sis”. These findings suggest, for the first time, that the variable breed is a factor potentially able to influence the fecal proteome in dogs.

INSTRUMENT(S):

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

SUBMITTER: daniela sorio

LAB HEAD: Cerquetella Matteo

PROVIDER: PXD066651 | Pride | 2025-09-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	LFQ_DALMATA_VS_WEIMARANER.msf	Msf
	Technical_Replicate_1_Dalmatians.raw	Raw
	Technical_Replicate_1_Weimaraners.raw	Raw
	Technical_Replicate_2_Dalmatians.raw	Raw
	Technical_Replicate_2_Weimaraners.raw	Raw

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Publications

Comparative Analysis of the Fecal Proteome in Two Canine Breeds: Dalmatians and Weimaraners.

Cerquetella Matteo M Pinnella Francesco F Morazzini Rachele R Rossi Giacomo G Marchegiani Andrea A Gavazza Alessandra A Mangiaterra Sara S Di Cerbo Alessandro A Sorio Daniela D Brandi Jessica J Cecconi Daniela D Vincenzetti Silvia S

International journal of molecular sciences 20250825 17

The analysis of proteins in stool samples can significantly enhance the study of mammalian physiology and disease. In this study, we investigated the fecal proteome of clinically healthy dogs (n = 26) by a label-free proteomics approach to evaluate the impact of breed differences. The dogs were divided into two groups (n = 13 each) based on their breed, specifically Weimaraner and Dalmatian, the former known for their possible susceptibility to gastrointestinal disease. Quantitative and qualitat ...[more]

PMID: 40943172

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