Tandem Mass Tag-Based Quantitative Proteomics of Extracellular Vesicles Isolated from Mammary Fibroblasts Treated with γ-radiation

ABSTRACT: Small extracellular vesicles (EVs) were isolated from a model of normal breast fibroblasts— immortalized cells derived from healthy reduction mammoplasty tissue—treated with γ-radiation to study the effects of radiation therapy on intercellular communication within the breast stroma. EVs were isolated using the gold-standard differential speed centrifugation protocol. Proteins were extracted and analyzed using a TMT-based quantitative proteomics approach.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fibroblast Of Breast, Breast

SUBMITTER: Kristie Lindsey Rose  

LAB HEAD: Kristie Lindsey Rose

PROVIDER: PXD066811 | Pride | 2026-06-01

REPOSITORIES: Pride