Proteomics

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Identifying Ceramide Protein Interactions using PISA

ABSTRACT: Ceramides are potent influencers of cell fate and function; however, their direct molecular target(s) and mechanism of action are poorly understood. In this study, we treated primary proximal tubule epithelial cells with C2-ceramide (50uM) or its precursor C2-dihydroceramide (50uM) and used proteome integral solubility alteration (PISA) to identify potential ceramide-specific protein effectors.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney Proximal Convoluted Tubule Epithelial Cell, Kidney

SUBMITTER: Rebekah Nicholson  

LAB HEAD: Scott Summers

PROVIDER: PXD067617 | Pride | 2026-06-08

REPOSITORIES: Pride

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Publications

Perturbation of proximal tubule (PT) lipid metabolism fuels the pathological features of acute kidney injury (AKI). We found that AKI induced biosynthesis of lipotoxic ceramides within PTs in humans and mice and that urine ceramides predicted disease severity in children and adults. Mechanistic studies in primary PTs, which included a thermal proteomic profiling screen for ceramide effectors, revealed that ceramides altered assembly of the mitochondrial contact site and cristae-organizing system  ...[more]

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