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Mesothelin Promotes Acute Myeloid Leukemia Cell Proliferation, Adhesion, and Chemoresistance via Novel Binding Partner Lyn

ABSTRACT: MSLN has only been implicated to have one binding partner, MUC16/CA125. In an effort to identifying novel binding partners of MSLN, an IP-MS experiment was conducted using immunoprecipitations of MSLN in whole cell lysate of NOMO-1 and MSLN KO cells. We identified Src-family kinase member Lyn, and guanine nucleotide-binding protein G(i), alpha subunit proteins, GNAI1, GNAI2, and GNAI3 as a novel binding partners of MSLN in AML.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Acute Myeloid Leukemia Cell Line

SUBMITTER: Joshua Faust

LAB HEAD: Sonali P. Barwe, PhD

PROVIDER: PXD067844 | Pride | 2026-04-27

REPOSITORIES: Pride

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			Action	DRS
	BarweS_100622.sdia	Other
	BarweS_100622_GPF6_1.mzML	Mzml
	BarweS_100622_GPF6_1.raw	Raw
	BarweS_100622_GPF6_2.mzML	Mzml
	BarweS_100622_GPF6_2.raw	Raw

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Publications

Mesothelin promotes acute myeloid leukemia progression through LYN-dependent signaling.

Faust Joshua R JR Hamill Darcy D Kolb E Anders EA Stevens Alexandra M AM Gopalakrishnapillai Anilkumar A Barwe Sonali P SP

The Journal of biological chemistry 20260320 5

Mesothelin (MSLN) is a glycosylphosphatidylinositol-anchored cell surface protein that is overexpressed in several solid tumors and in one-third of pediatric acute myeloid leukemia (AML) cases. It represents a validated immunotherapeutic target owing to its lack of expression in normal bone marrow. The function of MSLN in AML is unknown, but it is implicated to regulate adhesion in solid tumors through interaction with its only known binding partner, MUC16/CA125. This study uses CRISPR/Cas9 muta ...[more]

PMID: 41866035

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