Identification of Candidate Interaction Partners of Human Calpain-5 in Neuroblastoma Cells with SWATH-MS Analysis

ABSTRACT: Few interaction partners of human calpain-5/CAPN5 have been identified or proposed. This project was designed to identify candidate interaction partners of CAPN5 in SH-SY5Y neuroblastoma cells. We utilized sequential window acquisition of all theoretical mass spectra (SWATH‐MS) for proteomic analysis of samples resulting from co-immunoprecipitation with full-length CAPN5-3×FLAG. The bait was catalytically inactive due to a C81A mutation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Neuroblastoma Cell Line

DISEASE(S): Neuroblastoma

SUBMITTER: Carlos Gartner  

LAB HEAD: Calvin Vary

PROVIDER: PXD068008 | Pride | 2026-02-02

REPOSITORIES: Pride