Dataset Information

Proteome-wide characterization of FAS2 genomic elements swap

ABSTRACT: Protein synthesis is a highly regulated process that ensures the efficient assembly of protein complexes. Co-translational interactions are crucial to ensure the timely formation of functional protein complexes. One factor that can facilitate complex assembly is the spatial organization of mRNA within the cell. In this study we investigate the spatial distribution of mRNAs encoding for protein complex subunits in Saccharomyces cerevisiae, focusing on determining the co-localization state of these mRNAs and its impact on protein assembly and functionality. By swapping cis-regulatory elements upstream and downstream of the coding sequence we identified patterns that influence mRNA co-localization. We further quantified the resulting changes in global protein abundance in the swapped strains using LC-MS.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Rawad Hanna

LAB HEAD: Dr. Ayala Shiber

PROVIDER: PXD068196 | Pride | 2026-02-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Seq93125_88min_480Ex1.raw	Raw
	Seq93126_88min_480Ex1.raw	Raw
	Seq93127_88min_480Ex1.raw	Raw
	Seq93128_88min_480Ex1.raw	Raw
	Seq93129_88min_480Ex1.raw	Raw

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Publications

Distinct Cis-acting Elements Combinatorically Mediate Co-localization of mRNAs Encoding for Co-translational Interactors in Cytoplasmic Clusters in S. cerevisiae.

Shhade Qamar Q Estrada Marianna E ME Hanna Rawad R Makhzumy Muhammad M Bar-Yosef Hagit H Kramer Guenter G Bukau Bernd B Shiber Ayala A

Journal of molecular biology 20251114 2

Many newly synthesized proteins assemble co-translationally, providing a vital mechanism to prevent subunit misfolding in the crowded cytoplasm. Initial evidence indicates that the spatial organization of mRNAs aids this assembly, but it is unclear how these mRNAs are organized and how common this mechanism is. We used single-molecule Fluorescence in situ Hybridization in Saccharomyces cerevisiae to examine the spatial organization of mRNAs encoding subunits of various cytosolic complexes involv ...[more]

PMID: 41242541

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