Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation

ABSTRACT: Elg1, the major subunit of a Replication Factor C-like complex, is critical to ensure genomic stability during DNA replication, and is implicated in controlling chromatin structure. We investigated the consequences of Elg1 loss for the dynamics of chromatin re-formation following DNA replication. Measurement of Okazaki fragment length and the micrococcal nuclease sensitivity of newly replicated DNA revealed a defect in nucleosome re-assembly in the absence of Elg1. Using a proteomic approach to identify Elg1 binding partners, we discovered that Elg1 interacts with Rtt106, a histone chaperone implicated in replication-coupled nucleosome assembly that also regulates transcription. We find that Rtt106 recruitment to a number of promoters depends on Elg1. A central role for Elg1 is the unloadi

INSTRUMENT(S): NextSeq 500, AB SOLiD 4 System

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Sophie Shaw 

PROVIDER: E-MTAB-6985 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress