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Proteomic insight into alkane and 2-ketone degradation by Acinetobacter guillouiae FS11

ABSTRACT: Characterization of a commercial polyethylene (average Mw~4000, average Mn~1700) revealed the presence of low Mw alkanes and 2-ketones in the material. Based on gas-chromatography mass-spectrometry analyses, Acinetobacter guillouiae FS11 is able to metabolize these compounds. Proteomic analyses revealed the high abundance of enzymes involved in the alkane degradation pathway, as well as multiple flavin-containing monooxygenases (FMOs; putative Baeyer-Villiger monooxygenases), which may be utilized in the degradation of PE-derived compounds.

INSTRUMENT(S):

ORGANISM(S): Bacteria

SUBMITTER: Arturo Vera Ponce de Leon

LAB HEAD: Sabina Leanti La Rosa

PROVIDER: PXD068679 | Pride | 2025-12-04

REPOSITORIES: Pride

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			Action	DRS
	C30_planktonic_1_Slot2-4_1_480.d.tar.gz	Other
	C30_planktonic_2_Slot2-5_1_481.d.tar.gz	Other
	C30_planktonic_3_Slot2-6_1_482.d.tar.gz	Other
	C30_secreted_1_Slot2-16_1_492.d.tar.gz	Other
	C30_secreted_2_Slot2-17_1_493.d.tar.gz	Other

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Project description:We have developed a 60-mer oligonucleotide multibacterial microarray for detection and expression profiling of biodegradative genes and bacterial diversity (16S rRNA gene) in different habitats contaminated with varieties of hazardous chemicals. The genes selected were involved in biodegradation and biotransformation of various groups of compounds viz. nitroaromatic compounds (148 genes), chloroaromatic compounds (75 genes), monoaromatic compounds (373 genes), polyaromatic hydrocarbons (174 genes), pesticides/ herbicides (34 genes), alkanes/aliphatics (185 genes) and heavy metals (68 genes), which covered a total number of 133 chemicals. The efficiency (specificity, detection sensitivity) of the developed array was evaluated using the labeled genomic DNA of pure bacterial strains, Escherichia coli DH5M-NM-1 and Sphingomonas sp. strain NM-05 (involved in the biodegradation of M-NM-3-hexachlorohexane isolated from IPL, Lucknow) at different concentrations of 300ng, 500ng, 800ng, 1000ng and 1250ng. The specificity of the developed array was further validated using mixed cultures containing three strains (Sphingomonas sp. strain NM-05, Rhodococcus sp. strain RHA1 and Bordetella sp. strain IITR-02) involved in biodegradation of M-NM-3-hexachlorohexane, biphenyl and chlorobenzenes respectively. The mixed culture also contained non-target/non-degrader strains (E. coli DHM-NM-1, E.coli BL21 and E.coli K12 NCTC50192). The developed array was applied for profiling using the total soil DNA in five contaminated habitats of north India, viz. chloroaromatic chemicals contaminated site (India Pesticide Limited, Chinhat, Lucknow), a river sediments (Gomti river sediment, Lucknow), heavy metal industry dump site (Jajmau industrial area Kanpur), a effluent treatment plant (CETP along Ganges river near Kanpur), and an oil refinery (Mathura oil refinery). Hybridization of 16S rRNA probes revealed the presence of bacteria similar to well characterized genera involved in biodegradation of pollutants. Genes involved in complete degradation pathways for hexachlorocyclohexane (lin), 1,2,4-trichlorobenzene (tcb), naphthalene (nah), phenol (mph), biphenyl (bph), benzene (ben), toluene (tbm), xylene (xyl), phthalate (pht), Salicylate (sal) and resistance to mercury (mer) were detected with highest intensity. The most abundant genes belonged to hydroxylases, monooxygenases and dehydrogenases which were present in all the five samples. Many compound specific genes which initiate the degradation pathway were also detected. Thus, the array developed and validated here may be useful in assessing the biodegradative potential and composition of environmentally useful bacteria in hazardous ecosystems. Agilent one-color CGH experiment,Organism: Genotypic designed Agilent-17159 Genotypic designed Agilent Multibacterial 8x15k Array , Labeling kit: Agilent Genomic DNA labeling Kit (Part Number: 5190-0453)

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Proteomic insight into alkane and 2-ketone degradation by Acinetobacter guillouiae FS11

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