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MS-based quantitative proteomic analysis of UVB irradiation in XPC-deficient keratinocytes

ABSTRACT: Xeroderma Pigmentosum C (XPC) is a DNA damage recognition protein central to the global genome nucleotide excision repair (GG-NER) pathway, where it acts as a primary sensor of UV-induced DNA lesions. Loss-of-function mutations in the XPC gene lead to a photosensitive phenotype, with marked accumulation of unrepaired DNA damage and a dramatically elevated risk (10,000-fold) of skin cancer. This work aims at understanding the molecular signaling mechanisms associated with XP-C using genetically engineered human XPC knockout (KO) keratinocytes, the predominant cell type affected by UV radiation. We performed a mass spectrometry (MS)-based quantitative proteomic analysis, comparing XPC-wild-type and XPC-KO keratinocytes exposed or not to UVB for 24 hours.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratinocyte

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Couté

PROVIDER: PXD069309 | Pride | 2026-02-23

REPOSITORIES: Pride

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	Xpl1_004226.raw	Raw
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	Xpl1_004228.raw	Raw
	Xpl1_004229.raw	Raw
	Xpl1_004230.raw	Raw

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Publications

Type I interferon signaling defines a novel disease signature in xeroderma pigmentosum C human keratinocytes.

Nasrallah Ali A Rezvani Hamid-Reza HR Belmudes Lucid L Bourgoin-Voillard Sandrine S Kobaisi Farah F Fayyad-Kazan Mohammad M Seve Michel M Couté Yohann Y Sulpice Eric E Rachidi Walid W

Scientific reports 20260129

PMID: 41611895

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