Proteomics

Dataset Information

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HDX-MS analysis of NKp46 with next gen antibodies

ABSTRACT: Eptiopes of 7 unique antibodies produced by two complementary in vivo platforms utilizing a common light chain, in a chicken (OmniClic) or rat (OmniFlic) host species on NKp46 were analysed using HDX-MS. NKp46 was used as a model target antigen due to its use in emerging Natural Killer (NK) immune engagers that are being explored clinically as potentially safer alternatives to traditional CD3-based T cell engagers. We were able to successfully identify epitopes and map conformational changes of these antibodies validating our eptiope binning results from SPR.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: John Burke  

LAB HEAD: John E Burke

PROVIDER: PXD069455 | Pride | 2025-11-27

REPOSITORIES: Pride

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Dataset's files

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Action DRS
01-31-25_SS26_N10_10000s-1_17minElution.raw Raw
01-31-25_SS26_N11_10000s-1_17minElution.raw Raw
01-31-25_SS26_N_MSMS-200c-17min_elution.raw Raw
02-03-25_SS26_N10_1000s-1.raw Raw
02-03-25_SS26_N12_10000s-1_17minElution.raw Raw
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Publications

SH2-mediated steric occlusion of the C2 domain regulates autoinhibition of SHIP1 inositol 5-phosphatase.

Drew Emma E EE   Nyvall Hunter G HG   Parson Matthew A H MAH   Talus Reed K RK   Burke John E JE   Hansen Scott D SD  

The Journal of biological chemistry 20251007 12

The Src homology 2 (SH2) domain-containing inositol polyphosphate 5-phosphatase 1 (SHIP1) is an immune cell-specific enzyme that regulates phosphatidylinositol-(3,4,5)-trisphosphate signaling at the plasma membrane following receptor activation. SHIP1 plays an important role in processes, such as directed cell migration, endocytosis, and cortical membrane oscillations. Alterations in SHIP1 expression have been shown to perturb myeloid cell chemotaxis and differentiation. In the brain, SHIP1 regu  ...[more]

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