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Proteomic identification of GAP-binding proteins in colorectal cancer reveals PPP1CA as a direct target of microbial GABA-mediated metabolic rewiring

ABSTRACT: This dataset corresponds to the proteomic analysis presented in Figure 6A of the manuscript “Obesity-driven microbial GABA depletion promotes metabolic rewiring and colorectal cancer progression.” We performed Drug Affinity Responsive Target Stability (DARTS) and co-immunoprecipitation proteomics using colorectal cancer cell lysates incubated with glyceraldehyde-3-phosphate (GAP) to identify GAP-binding proteins. Mass spectrometry data revealed PPP1CA as a key GAP-interacting protein mediating GABA-dependent suppression of YAP signaling. The dataset supports the mechanistic link between microbiota-derived GABA, TPI1-generated GAP, and YAP-regulated tumor metabolism in obesity-associated colorectal cancer.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Dawei Chen

LAB HEAD: Jingxin Li

PROVIDER: PXD069744 | Pride | 2026-06-21

REPOSITORIES: Pride

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			Action	DRS
	RKOCon1.msf	Msf
	RKOCon1.raw	Raw
	RKOCon2.msf	Msf
	RKOCon2.raw	Raw
	RKOCon3.msf	Msf

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