The proteomics analysis of mitochondria-enriched fractions reveals alteration of key mitochondrial pathways in breast cancer cell lines

ABSTRACT: Breast cancer is the leading cause of death in women worldwide. Plasticity and heterogeneity of breast cancer cells make this disease extremely difficult to treat. Many reports point to metabolic shifts, mainly those carried out in o mitochondria, as key processes governing the behavior and heterogeneity of several types of breast cancer. In this study, we performed label-free proteomics analysis on mitochondria-enriched fractions from T47D and MDAMB-231 breast cancer cell lines, which have distinct molecular classifications, using bioinformatics analyses to identify differentially expressed proteins compared to MCF-12F healthy breast cells. Cancer cells exhibited down-regulated protein levels of subunits from the respiratory chain's Complex I. However, both presented differentially abundant proteins related to ligase and oxidoreductase activities, such as enzymes involved in glycolysis, pyruvate metabolism, the Krebs cycle, and gluconeogenesis. Many of these enzymes also participate in other metabolic processes, such as localization to the mitochondrion, mitochondrial gene expression, and the metabolism of amino acids, fatty acids, purines, and pyrimidines. Gene Set Enrichment Analysis revealed that OXPHOS enzymes play key roles in many neurodegenerative disease pathways. A protein set with little or no evidence in breast cancer was identified, which could lead to future research in breast cancer mitochondrial metabolism.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Breast Cancer Cell Line

DISEASE(S): Breast Cancer

SUBMITTER: Rodolfo Moreno Castillo  

LAB HEAD: Juan Pedro Luna-Arias

PROVIDER: PXD069883 | Pride | 2026-04-27

REPOSITORIES: Pride