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Conformational dynamics of CagM homo-oligomerization by HDX-MS

ABSTRACT: Cag type IV secretion system (CagT4SS) mediates the translocation of a wide spectrum of substrates including oncoprotein CagA to host cells and plays an indispensable role in the pathogenesis of Helicobacter pylori. CagM, one of the central cylinder components responsible for initiating the assembly of CagT4SS, is able to undergo high-order homo-oligomerization. Two CagM mutants, NTD-EK (E29K/K31E/K32E/E33K/E35K/E36K) and DEK (D183E/E184K/E185K), deficient in high-order oligomerization were generated. Here, we performed differential HDX-MS analysis on wild-type, to characterize the interface involved in homo-oligomerization. Our findings reveal the significance of CagM homo-oligomerization on facilitating CagT4SS assembly.

INSTRUMENT(S):

ORGANISM(S): Helicobacter Pylori G27

SUBMITTER: Chin Yu Mok

LAB HEAD: Shannon Wing Ngor Au

PROVIDER: PXD075969 | Pride | 2026-06-14

REPOSITORIES: pride

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			Action	DRS
	CagM_NTD-EK_DEK_raw.zip	Other
	SuppTable4_CagM_NTD-EK.xlsx	Xlsx
	SuppTable5_CagM_DEK.xlsx	Xlsx
	checksum.txt	Txt

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