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Homo sapiens strain:HaCaT

ABSTRACT: RNA-seq, 5H4PB treated human keratinocyte cell line HaCaT

PROVIDER: PRJDB13297 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR357080_1.fastq.gz	Fastqsanger.gz
	DRR357080_2.fastq.gz	Fastqsanger.gz
	DRR357081_1.fastq.gz	Fastqsanger.gz
	DRR357081_2.fastq.gz	Fastqsanger.gz
	DRR357082_1.fastq.gz	Fastqsanger.gz

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Homo sapiens strain:HaCaT

Project description:RNA-seq, LA treated human keratinocyte cell line HaCaT

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Gene expression profile at single cell level of HaCaT cells exposed to ionizing radiation

Project description:HaCaT cell line is a kind of keratinocyte from human skin. We used single cell RNA sequencing (scRNA-seq) to analyze the influnce of fractionated 2 Gy or single 20 Gy irradiation on HaCaT cells.

2022-12-31 | GSE210170 | GEO

Human keratinocytes HaCaT LC-MS/MS

Project description:Triton X-100 has been used as a model substance to study the effects of irritants on the skin. HaCaT human keratinocyte cell line were treated with a non-cytotoxic dose of Triton X-100, as determined by the MTT assay and microscopically examination) for 48 h. The altered abundance of proteins from HaCaT keratinocytes exposed to Triton X-100 was analyzed by LC-MS/MS approach and quantified using Progenesis LC software.

2017-11-03 | PXD007776 | Pride

Methylated RNA immunoprecipitation sequencing (MeRIP-seq) of human epidermis and HaCAT cells with or without ALKBH5 knockdown

Project description:We performed MeRIP-seq on (1) two human epidermis tissue and HaCAT (human keratinocyte cell line) cells; (2) HaCAT cell lines with or without ALKBH5 knockdown by siRNA in duplicate experiments.

2022-08-18 | GSE211442 | GEO

HaCaT keratinocytes day3 vs HaCaT keratinocytes day10

Project description:The keratinocyte cell line HaCaT was cultured for three days (proliferation) or for ten days (differentiation). RNA from cells at day3 was compared to RNA from cells at day10. The microarray hybridizations were performed in dye-swap procedure : RNA from cells at day3 was labeled with Cy3 (GSM4674, GSM4675, GSM4682, GSM4683) and then with cy5 (GSM4680, GSM4681). Keywords = cell density differentiation program in human keratinocytes

2003-03-10 | E-GEOD-336 | biostudies-arrayexpress

HaCaT keratinocytes day3 vs HaCaT keratinocytes day10

2003-03-11 | GSE336 | GEO

Study of transcriptional effects of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line.

Project description:Aim of the study was to characterize at a molecular level (changes in transcriptomes) the effect of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line. This was adressed by using a culture model. The HaCaT cell line (human keratinocytes) was stimulated by MSU (1mg/mL) vs control for 12 hrs. By using genome-wide expression profiling, we identified deregulation of functionally relevant gene networks. HaCaT were obtained from Cell Lines Service (Eppelheim, Germany) and grown in DMEM medium (PAN biotech, Aidenbach, Germany) supplemented with 10% FBS (Life Technology, Grand Island, NY, USA), L-glutamine and non-essential amino acid. Before the treatment HaCaT cells were cultured in serum-free medium for 12hrs. HaCaT were treated with MSU (1mg/ml) vs DMEM control for 12hrs then submitted to RNA extration and gene expression profiling. Triplicate experiments were performed: HaCaT control (n=3), MSU-treated (n=3).

2015-10-29 | E-GEOD-65931 | biostudies-arrayexpress

To investigate the alteration of gene expression profiles after NCSTN gene silencing in HaCaT cells employing Agilent whole genome microarray

Project description:The present study was designed to use small interfering RNA (siRNA) approach to identify the effects of NCSTN silencing on human immortal keratinocyte cell line, HaCaT. The alteration of gene expression profiles after NCSTN gene silencing in HaCaT cells was investigated employing Agilent whole genome microarray

2018-04-28 | GSE68346 | GEO

RNA-seq in human keratinocytes HaCaT cell line

Project description:RNA-seq in human keratinocytes HaCaT cell line

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Protein dataset of immortalized HaCaT cells p53 knock down HaCaT keratinocytes

Project description:The p53 protein is encoded by TP53 gene and plays the key role in significant number of cellular processes including proliferation, apoptosis and regulation of many stress response pathways. P53 acts like a direct transcription activator of numerous genes regulating cell cycle arrest, DNA repair, growth inhibition and many others (Mollereau and Ma, 2014). The canonical biological function of p53 is maintaining genome integrity via elimination of damaged or exposed to genotoxic stress cells. Immortalized HaCaT cells are widely used for keratinocyte research, since they maintain stable keratinocyte phenotype, have nearly unlimited proliferative potential, do not require specific growth and differentiation factors (Colombo et al., 2017). Also, HaCaT cells produce typical differentiation markers such as cytokeratins K14 and K10, involucrin (Colombo et al., 2017) and respond to keratinocyte differentiation stimuli. Taking together, HaCaT cells have similar to normal human keratinocytes (NHK) properties, however, as many of spontaneously immortalized cell lines HaCaT cells bear two mutant p53 alleles - R282Q and H179Y (Lehman et al., 1993). Mutp53 in HaCaT has an increased affinity to other p53 family members (p63, p73), which significantly expands p53 properties. Moreover, mutp53 indirectly affects specific target genes via protein-protein interactions with other transcription factors (NF-Y, E2F1, NF-KB) or by tethering p63 to new promotor locations. For more detailed investigation of mutp53 impact on various processes in HaCaT cells we performed a shRNA mediated knockdown of mutp53. For generation of stable TP53 knockdown we employed plasmid vector pLKO-p53-shRNA-941 (Addgene # #25637) followed by puromycin selection of transduced cells. Here we present proteomic dataset obtained from wild type HaCaT cells and p53 knock down HaCaT keratinocytes.

2022-04-28 | PXD033538 | Pride

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