ABSTRACT: Complete genome sequence of Bacillus velezensis RB.IBE29, the chitinolytic bacterium isolated from the rhizospheric soil of black pepper cultivated in the Central Highlands, Vietnam
Project description:To study microbial interaction effect on transcriptomic profile of B. velezensis 83, a comparative gene expression profiling analysis was performed.
2025-05-10 | GSE202842 | GEO
Project description:Analysis of endophytic microbiome dataset from roots of black pepper (Piper nigrum L.) cultivated in the Central Highlands region, Vietnam using 16S rRNA gene metagenomic next-generation sequencing
Project description:In this study, we used the illumina high throughput sequencing approach (Sequencing-By-Synthesis, or SBS) to develop the sequence resource of black pepper. To identify micro RNAs functioning in stress response of the black pepper plant, small RNA libraries were prepared from the leaf and root of Phytophthora capsici infected plants, leaves from drought stressed and control plants.
Project description:The present study aims to evaluate the response of the three Mediterranean local grapevines ‘Garnacha Blanca’, ‘Garnacha Tinta’, and ‘Macabeo’ to treatments with biocontrol products (BPs), a botanical extract (Akivi, Dittrichia viscosa extract) and a beneficial microorganism (Bacillus UdG, Bacillus velezensis). A combination of transcriptomics and metabolomics approaches were chosen in order to study grapevine gene expression and to identify gene marker candidates, as well as, to determine grapevine metabolites differentially concentrated in response to BPs treatments. Grapevine plants were cultivated in greenhouse controlled conditions and submitted to the treatments, and thereafter, leaves were sampled 24h after treatment to conduct gene expression study by RNA-sequencing for ‘Garnacha Blanca’ leaves extract and by RT-qPCR for the three cultivars. Differentially expressed genes (DEGs) were investigated for both treatments and highly influenced DEGs were selected to be tested in the three cultivars as treatment gene markers. In addition, extraction of leaf components was performed to quantify metabolites such as phytohormones, organic acids, and phenols. Considering all the upregulated and downregulated genes and enhanced metabolites concentrations, the treatments had an effect on jasmonic acid, ethylene, and phenylpropanoids defense pathways. In addition, several DEG markers were identified presenting a stable overexpression after the treatments in the three grapevine cultivars. These gene markers could be used to monitor the activity of the products in field treatments in future research. Further research will be necessary to confirm these first results under field conditions.
2022-12-18 | GSE211268 | GEO
Project description:Microbial diversity in cultivated and wild Black pepper
| PRJNA1330850 | ENA
Project description:Microbial diversity in cultivated and wild Black pepper
Project description:Untargeted proteomics of full protein extract of Bacillus velezensis P45 in prebiotics and mucin cultures
| MSV000099225 | MassIVE
Project description:Root endophytic microbiome dataset of sugarcane (Saccharum officinarum L.) cultivated in the Central Highlands, Vietnam, established by the 16S rRNA metagenomics
