Project description:We performed shotgun proteomics on the bacteria Prevotella brevis GA33 and Prevotella ruminicola 23. We did this for two types of samples (cell extract and cell membrane) and using two methods (data-dependent and data-independent acquisition).
Project description:The transcriptional responses of Prevotella ruminicola were determined during growth on 20 different carbohydrate sources including mono-, di- and tri- saccharides, oligosaccharides, polymeric xylan and complete plant material with the aim of obtaining a more complete understanding of the number of genes and metabolic networks associated with carbohydrate catabolism by this organism All microarray hybridizations and subsequent scanning was undertaken using a two colour system (Cy3 and Cy5): 34 samples
Project description:Anaerobic bacteria in the oral cavity can cause respiratory infections. However, their precise mechanisms of action remain elusive. Unexpectedly, bacterial flora analysis using 16s rRNA revealed ‘hidden’ mixed infections of anaerobic bacteria and commensal oral Streptococcus species in community-acquired pneumonia. The purpose of this study is to elucidate the mechanisms by which Prevotella intermedia exacerbates oral streptococcal pneumonia.
Project description:Spontaneous chylothorax is rare in adults. We present an unusual case that was complicated by Prevotella bivia empyema. Full recovery was achieved with chest tube drainage and prompt treatment with intravenous clindamycin.
Project description:Prevotella bryantii B14 was cultivated with monensin. Growth was monitored over a period of 9h with a broad range of monensin concentrations.
