Project description:We used DNA microarrays to detect differentially expresses genes in a spontaneous mutant of Mycobacterium tuberculosis H37Rv resistant to azoles. We identified three adjacent upregulated genes encoding a transcription regulatory protein and two membrane proteins belonging to the family of RND efflux transporters.
Project description:We used DNA microarrays to detect differentially expresses genes in a spontaneous mutant of Mycobacterium tuberculosis H37Rv resistant to azoles. We identified three adjacent upregulated genes encoding a transcription regulatory protein and two membrane proteins belonging to the family of RND efflux transporters. Hybridization were performed using RNA extracted from three different biological samples coming from cultures grown in mid-exponential phase.
Project description:Efflux pump overexpression is associated with drug resistance in Mycobacterium tuberculosis, but its role in delamanid resistance is not well understood. This study sought to identify efflux pump genes affected by delamanid exposure using transcriptomic analysis. Cultures of M. tuberculosis H37Rv were exposed to delamanid at a sub-inhibitory concentration of ½ MIC for 24 hours, and transcriptomic sequencing was conducted on both treated and untreated samples. Differentially expressed genes (DEGs) were annotated using the UniProt database. The analysis revealed significant upregulation of 23 efflux pump genes under delamanid stress, with 73.9% belonging to the ABC family, 17.39% to the RND family, and 8.69% to the MFS superfamily. These results highlight efflux pump genes that are responsive to sub-inhibitory levels of delamanid.
Project description:Mycobacterium tuberculosis is a pathogenic bacterium that was sequenced and annotated as early as 1998, but in recent years it has been found that its annotation is not complete. Therefore, we used a proteogenomics strategy to re-annotate Mycobacterium tuberculosis H37Rv, identifying 37 'new genes' and correcting 35 existing gene models.
