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Deep sequencing of human naive CD4 T cells identifies miR-34c-5p as a novel regulator of T cell activation and HIV replication

ABSTRACT: Deep sequencing of human naive CD4 T cells identifies miR-34c-5p as a novel regulator of T cell activation and HIV replication

PROVIDER: PRJEB18611 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1758000.fastq.gz	Fastqsanger.gz
	ERR1757981.fastq.gz	Fastqsanger.gz
	ERR1757991.fastq.gz	Fastqsanger.gz
	ERR1758011.fastq.gz	Fastqsanger.gz
	ERR1758002.fastq.gz	Fastqsanger.gz

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Deep sequencing of human naive CD4 T cells identifies miR-34c-5p as a novel regulator of T cell activation and HIV replication

Project description:Cell activation is a vital step for T cell memory/effector differentiation as well as for productive HIV infection. To identify novel regulators of this process, we used next generation sequencing to profile changes in microRNA expression occurring in purified human naive CD4 T cells in response to TCR stimulation and HIV infection. HIV infection had no significant impact on global miR expression in quiescent nave CD4 T cells. We identified miR-34c-5p as a novel miR strongly induced by TCR stimulation of nave CD4 T cells, and found that it was consistently down-regulated in response to viral infection. Over-expression of miR-34c-5p had a positive effect on HIV-1 replication. Finally, we demonstrated that miR-34c-5p alters the expression of several genes involved in TCR signaling and cell activation, identifying it as a novel regulator of nave CD4 T cell activation potentially targeted by HIV infection.

2016-12-21 | E-MTAB-4214 | biostudies-arrayexpress

Deep sequencing of human naive CD4 T cells identifies miR-34c-5p as a novel regulator of T cell activation and HIV replication

Project description:We generated a model for miR-34c-5p overexpression by transfecting Jurkat T cell line with a pcDNA3 vector containing the pre-miR-34c-5p precursor sequence and selecting for antibiotic resistance to isolate both pools and clones of stably transfected cells (J34c cells), in parallel with control cells transfected with the empty parental vector (JÃ cells). Both pools of stably transfected cells and the derived clonal lines expressed high levels of the miR.

2016-12-21 | E-MTAB-4190 | biostudies-arrayexpress

Identification of miR-34c-5p as a potential regulatory molecule associated with testicular development of black goat

Project description:The growth and mature of Sertoli cells (SCs) are important part of testicular development and miRNAs are considered as novel regulatory molecules involved in it. Here we systematically screened and analyzed the key miRNAs participating in testicular development of black goat, as well as identifying underlying mechanism of candidate mRNAs contributes to spermatogenesis. The miRNA transcriptional profiles of testicular tissues at 3 days old (D3), 30 days old (D30), 90 days old (D90) and 180 days old (D180) were constructed and comparative analyzed. Results showed that the most differentially expressed (DE) miRNAs were found between the testicular tissues of D30 and D90 black goats. Moreover, the predicted target genes of DE miRNAs are mainly enriched in biological processes including cell proliferation, cell composition and enriched in signaling pathways including p53, FoxO and PI3K-Akt. The top one DE miRNA (miR-34c-5p), which significantly highly expressed in the testis of D90 when compared to that of D30, was mainly located within SCs and targeted to IGF1R. By performing miR-34c-5p overexpression and RNA interference, we found that the function of miR-34c-5p in SCs was inhibited cell proliferation, increased apoptosis rate, and significantly reduced the expression of growth factors (CXCL12, BMP4, GDNF and IGF1). In summary, our study suggests that miR-34c-5p can serve as a potential target for regulation of testicular development and a reference marker of sex maturation applicated in black goat genetic breeding.

2025-10-01 | GSE279238 | GEO

miRNA profiling of human naive CD4 T cells links miR-34c-5p to cell activation and HIV replication.

Project description:Cell activation is a vital step for T-cell memory/effector differentiation as well as for productive HIV infection. To identify novel regulators of this process, we used next-generation sequencing to profile changes in microRNA expression occurring in purified human naive CD4 T cells in response to TCR stimulation and/or HIV infection. Our results demonstrate, for the first time, the transcriptional up-regulation of miR-34c-5p in response to TCR stimulation in naive CD4 T cells. The induction of this miR was further consistently found to be reduced by both HIV-1 and HIV-2 infections. Overexpression of miR-34c-5p led to changes in the expression of several genes involved in TCR signaling and cell activation, confirming its role as a novel regulator of naive CD4 T-cell activation. We additionally show that miR-34c-5p promotes HIV-1 replication, suggesting that its down-regulation during HIV infection may be part of an anti-viral host response.

| S-EPMC5286376 | biostudies-literature

Assessing the impact of miR-34c in CD4+ T cell transcriptome

Project description:Transcriptome profile of TCR-stimulated naive CD4+ T cells treated with miR-34c-5p-specific versus control antagomiRs

| PRJEB87428 | ENA

Identification of miR-34c targets

Project description:In this study we set out to characterize the mechanisms by which miR-34c deregulation contribute to PCa progression. The genes regulated by miR-34c in the prostate cancer cell line PC3 were identified by microarray analyses, and the top biofunctional pathways enriched for identified genes were found to be cell death, cell cycle, cellular growth, and cellular movement. One of the identified targets was MET, a receptor protein tyrosine kinase activated by hepatocyte growth factor, that is crucial for metastatic progression. see above

2013-12-01 | GSE41322 | GEO

Identification of miR-34c-5p as a potential regulatory molecule associated with testicular development of black goat

Project description:Identification of miR-34c-5p as a potential regulatory molecule associated with testicular development of black goat

| PRJNA1171457 | ENA

A global transcriptome analysis of human epidermal keratinocytes upon overexpression of microRNA-34a or microRNA-34c

Project description:miR-34a and miR-34c were found up-regulated at wound-edges of human venous ulcer compared to nomal wound and the intact skin; however their biological role in keratinocytes during wound repair has not been studied. To study the genes regulated by miR-34a and miR-34c, we transfected miR-34a and miR-34c mimic into human primary epidermal keratinocytes to overexpress them. We performed a global transcriptome analysis of keratinocytes upon overexpression of miR-34a or miR-34c using Affymetrix arrays.

2019-09-04 | GSE117506 | GEO

Gene expression changes initiated by miR-34c in Dicer/Pten double knockout mouse serous epithelial cancer

Project description:miR-34c inhibits Dicer/Pten double knockout mouse serous epithelial cancer cell proliferation by inducing cell cycle arrest and apoptosis. We found that miR-34c had a more dramatic effect on inhibiting tumor cell viability than let-7b. The action of miR-34c induced tumor cell cycle arrest in G1 phase and apoptosis and was accompanied with the regulation of key genes involved in cell proliferation and cell cycle G1/S transition. miR-34c suppressed the expression of EZH2 and MYBL2, which may transcriptionally and functionally activate CDKN1C.

2014-10-03 | GSE57493 | GEO

Gene expression changes initiated by miR-34c in Dicer/Pten double knockout mouse serous epithelial cancer

2014-10-03 | E-GEOD-57493 | biostudies-arrayexpress