Project description:Sulfoquinovose (SQ) is a major organosulfonate in nature, and thus plays an important role in the biogeochemical sulfur and carbon cycles. We identified a bacterial anaerobic consortium, enriched from lake Konstanz, which degraded SQ to isethionate as intermediate and further into acetate and sulfide. By a metagenomic analysis we identified Faecalicatena sp. DSM22707 as major SQ-degrader in the consortium. Strain DSM22707 degraded SQ in pure culture into isethionate and small amounts of sulfolactate.
2025-05-07 | PXD055296 | Pride
Project description:E. cheiranthoides F2 population from cross between Elbtalaue and Konstanz accessions
Project description:We performed a transcriptome profiling on the leaf 3 microdissected from plants with altered cytokinin levels 3h after activation at 9 DAS, when this leaf is normally fully composed of proliferating cells to get an insight into dynamics of molecular network underlying cell responses to CK excess and deficiency in proliferating cells. Seeds of Arabidopsis thaliana transgenic lines (CaMV35S>GR>ipt and CaMV35S>GR>HvCKX2) and corresponding wild-types (Col-0) were sown on nylon meshes placed on half-strength Murashige and Skoog (MS) medium containing 0.5% (w/v) MES and 0.8% (w/v) agar. After 2 days of stratification, plants were removed to growing chambers with 16-h day/8-h night, 21 C and 19 C, respectively, under continuous light (110 mol m-2 s-1). DEX-inducible lines and wild-types were activated at 9 DAS by transfer of nylon meshes to half-strength MS medium supplemented with either 2.5 M DEX dissolved in 5x10-4% (v/v) DMSO or just 5x10-4% (v/v) DMSO (mock). The leaf 3 was dissected from i > 250 individual seedlings per variant on a cooling plate under a stereomicroscope with precision needle and removed into the liquid nitrogen. For RNA-seq 2 biological replicates of WT, ipt and HvCKX2 activated by DEX were used. The RNA was extracted according the same method described for qRT-PCR. RNA sequencing was done by GATC Biotech AG (Konstanz, Germany).
Project description:Activated sludge bacterial and archeal communities from Konstanz, Germany to study Microbial Dark Matter (Phase II) - J533_phenol metaG metagenome
Project description:Intra- and extracellular metabolomics dataset of human dermal blood endothelial cells (HDBECs), human umbilical vein endothelial cells (HUVECs), human dermal lymphatic endothelial cells (HDLECs) and intestinal lymphatic endothelial cells (iLECs) in proliferation and quiescence.