Project description:The Imitation Switch (ISWI) chromatin remodeling factors are known to form stable remodeling complexes with members from a family of proteins, termed DDT-domain proteins. ISWIs have been reported to be involved in various biological processes in many eukaryotic species. However, the regulatory mechanisms of DDT-domain proteins in ISWI complexes remain unclear. In this study, using ChIP-seq assays, we found that FgDDT binds to the promoter of MAPK and growth-related gene, activating its expression. Moreover, we identified and characterized two additional ISWI genes, FgISW1 and FgISW2, indicating that the ISWI components FgISW1 and FgDDT, rather than FgISW2, are essential for fungal development and virulence. Subsequently, we illustrated that FgDDT binds to FgISW1 to form an ISWI complex that activates the expression of functional genes in Fusarium graminearum, contributing to the virulence and development of F. graminearum. We also we observed that FgDDT is highly conserved in Fusarium species, but exhibits low similarity in Homo sapiens and Arabidopsis thaliana. These findings provide a basis for further studies on the molecular mechanisms by which ISWI complexes function in fungi.
Project description:Role of fungal cellulases upon Fusarium oxysporum infection. We obtained Fusarium oxysporum mutants, which cannot degrade cellulose capacity to observe their virulence. Cellulose degradation is not mandatory for Fusarium oxysporum to reach the plant vasculature system.
Project description:Fusarium graminearum (F.g) is responsible for Fusarium head blight (FHB), which is a destructive disease of wheat that accumulates mycotoxin such as deoxynivalenol (DON) and makes its quality unsuitable for end use. Several FHB resistant varieties development is going on world-wide. However the complete understanding of wheat defence response, pathogen (Fusarium graminearum) disease development mechanism and the gene crosstalk between organisms is still unclear. In our study focused to analyse pathogen (F. graminearum) molecular action in different Fusarium head blight resistance cultivars during the disease development. To understand the Fusarium graminearum pathogen molecular reaction, microarray gene expression analysis was carried out by using Fusarium graminearum (8 x 15k) Agilent arrays at two time points (3 & 7 days after infection) on three wheat genotypes (Japanese landrace cv. Nobeokabouzu-komugi - highly resistant, Chinese cv. Sumai 3 - resistant and Australian cv. Gamenya - susceptible), which spikes infected by Fusarium graminearum ‘H-3’strain. During the disease development the pathogen biomass as well as the expression of Trichothecene biosynthesis involved genes (Tri genes) in three wheat cultivars was determined. In our material no relation between fungus biomass and the disease symptoms were observed, however, it showed relation with fungus virulence factors expression (Tri genes). For the first time, we report the nature of Fusarium graminearum gene expression in the FHB-highly resistant cv. Nobeokabouzu-komugi during the disease development stage and the possible underlying molecular response.
Project description:The analysis of the Fusarium graminearum transcriptome during symptomless and symptomatic infections of wheat floral tissue at 7 days post infection, to identify the correct spatio-temperol regulation of multiple virulence strategies.