Ontology highlight
ABSTRACT:
PROVIDER: PRJEB27219 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Seeds, as an effective depository of plant genetic resources and an important raw material for germplasm innovation, aging or deterioration will directly lead to a reduction in germination rate, vigor and viability, inhibit the normal development of the seed embryo and seedling growth. Methods: Allium mongolicum seeds stored naturally for 2, 4 and 5 years were used as test materials, determination of germination indexes, antioxidant enzyme activities and other physiological indexes in seeds treated with 0.8 mmol/L spermidine solution, and proteomic and transcriptomic sequencing were performed. Results: The results showed that the germination ability of Allium mongolicum seeds decreased continuously with the extension of storage life, and the germination rate was only about 40% after 5 years of storage. The relative conductivity, MDA and H2O2 content were significantly higher, CAT, POD, SOD, APX, and respiratory rate were significantly reduced; Spd treatment increased seed germination rate, significantly reduced MDA, H2O2 content and relative conductivity, and increased CAT, POD, SOD, and APX activities. Functional analysis of the differentially expressed proteins showed that the differentially expressed proteins were mainly concentrated in energy metabolism pathways such as carbon metabolism, amino acid biosynthesis, glycolysis/glycogen isomerization, TCA cycle, pyruvate metabolism and pentose phosphate metabolism. Transcriptome sequencing yielded similar results. PPI analysis identified 10 proteins related to the aging of Allium mongolicum seeds, and the core protein was also verified by qPCR, the gene expression trend was basically consistent with the proteomic assay results. Conclusions : Energy metabolism may be a key pathway in the aging of Allium mongolicum seeds, and spermidine can effectively alleviate Allium mongolicum seed aging. The results enriched the molecular mechanism of seed storability of Allium mongolicum and provided theoretical basis for molecular marker-assisted breeding of storability traits in Allium mongolicum L.
2024-11-18 | PXD058020 |
Project description:Purpose: Aim of this study was to determine the miRNAs associated with average daily gain in Kangal Akkaraman lambs. Depending on the average daily gain, two groups were formed as low average daily gain (LDG) and high average daily gain (HDG). Methods: Peripheral blood mononuclear cells (pbmc) were separated from blood samples obtained from lambs and RNA was isolated with TRI Reagent (Sigma). Isolated total RNAs from LDG (n = 17) and HDG (n = 21) groups were pooled. miRNA libraries (LDG and HDG groups) were prepared with NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) and sequenced with Illumina Novaseq. Readings were mapped with the Bowtie program using the Oar_v3.1 sheep reference genome. Differential expression analysis of the two groups was performed using the DESeq R package (1.8.3). Results: Using an optimized data analysis workflow, we mapped about 18 million sequence reads in HDG and 21 million sequence reads n LDG samples to the ovis aries genome (oar_v3.1). In total, 129 known miRNAs were identified, 134 novel miRNAs were predicted, and 40 differentially expressed miRNAs were found between the groups. Conclusions: Our study represents the first detailed analysis of Kangal type Akkaraman sheep PBMC miRNAs with RNA-seq technology. The obtained sequence reads would provide data for futher breeding studies related to average daily gain in Kangal Akkraman sheep.
2021-03-11 | GSE168662 | GEO
Project description:Allium mongolicum, a xerophytic industrial plant native to the Mongolian Plateau, demonstrates notable stress resistance, though its salt-alkali tolerance mechanisms remain poorly understood. This study integrates physiological assessments, transcriptomics, metabolomics, and full-length transcriptome analyses to uncover its adaptive mechanisms under salt-alkali stress.A comprehensive full-length transcriptome was constructed under these conditions, comprising 30,117 non-redundant genes, alongside significant trends observed in the activities of antioxidant enzymes and key enzymes in the phenylpropanoid pathway. Combined transcriptomic and metabolomic analyses identified key genes and metabolites in the phenylpropanoid pathway as central to salt-alkali tolerance. WGCNA further highlighted critical genes, such as AmCOMT1, AmHSP18, and AmPPL7, with functional validation of AmCOMT1 and AmHSP18 confirming transcriptome reliability. The salt tolerance function of AmCOMT1 has been validated through overexpression in plants, and the binding interaction with AmERF4 has also been confirmed.A proposed model suggests A. mongolicum mitigates salt-alkali stress via reactive oxygen species scavenging, osmotic regulation, and structural support, providing valuable insights for breeding salt-tolerant crops and enhancing its agricultural applications.
2026-02-08 | GSE289259 | GEO
Project description:Transcriptome Analysis of Allium mongolicum Regel: Drought Resistance Comparison Between Wild (Minqin, GS) and Domesticated (Tongliao, XH) Accessions
| PRJNA1277023 | ENA