Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell RNA-seq of WTC-CRISPRi human induced pluripotent stem cells

ABSTRACT: Single cell RNA-seq of WTC-CRISPRi human induced pluripotent stem cells

PROVIDER: PRJEB28253 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Metabolomics Workbench ST000003 - GNPS - Metabolomic analysis of mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells

Project description:mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells were compared via metabolomic analysis

2018-08-02 | MSV000082680 | GNPS

CRISPRi/a screen of doxorubicin induced cardiotoxicity

Project description:Druggable genome of CRISPRi/a sgRNA libraries for druggable genes protecting doxorubicin induced cardiomyocyte toxicity using human induced pluripotent stem cell (iPSC) derived cardiomyocytes.

2024-09-01 | GSE276161 | GEO

Hyperosmolarity stress associated proteomic changes in induced pluripotent stem cells.

Project description:Comparison of hyperosmolarity stress associated proteomic changes between induced pluripotent stem cells (hiPSCs/IMR90-01) and differentiated SH-SY5Y cells.

2020-10-23 | MSV000086363 | MassIVE

Human induced pluripotent stem cell differentiation to cardiomyocytes

Project description:Shotgun proteomics analysis of directed differentiation of human induced pluripotent stem cells to cardiomyocytes

2019-12-16 | PXD013426 | Pride

Single-cell RNA-seq of CRISPRi ADGRG6-kd in iAT2

Project description:We performed single cell transcriptomic profiling of induced human pluripotent stem cells (iPSCs)-derived type 2 alveolar epithelial cells (iAT2). iPSCs stably expressed CRISPRi (dCas9-KRAB) under the control of doxycyline. iAT2s were transduced with a lentivirus expressing gRNA targeting the transcriptional start site of ADGRG6. Cells were treated with or without doxycyline to intiate CRISPRi-knockdown. Prior to capture, cells were labelled with hashing antibodies (HTO). Cells were captured for 10x Genomics Single cell capture (V3 protocol), and GEX and HTO libraries were sequenced. HTODemux function was used to demultiplex the samples. Knockdown of ADGRG6 caused cells to cluster separately from wild-type cells.

2023-09-20 | GSE223078 | GEO

N-glycoproteomics of induced pluripotent stem cells, embryonic stem cells, and parental cells

Project description:We tried to understand the N-glycoproteom molecular differences between induced pluripotent stem cells and embryonic stem cells. This is important to improve the reprograming process and induced pluripotency in the context of post translational protein N-glycosylation

2016-12-23 | PXD004366 | Pride

Deep single-cell proteomics quantifies low-abundant embryonic transcription factors and lineage markers during induced pluripotent stem cell differentiation

Project description:Single-cell proteomics (SCP) promises to revolutionize biomedicine by providing an unparalleled view of the proteome in individual cells. Here, we present a high-sensitivity SCP workflow identifying >5000 proteins in individual HeLa cells. It also facilitated direct detection of post-translational modifications (PTMs) in single-cells, negating the need for specific PTM-enrichment. Our study demonstrates the feasibility of processing up to 80 label-free SCP samples per day. An optimized tissue dissociation buffer enabled effective single cell disaggregation of drug-treated cancer cell spheroids, refining overall SCP analysis. Analyzing non-directed induced pluripotent stem cell (iPSC) differentiation, we consistently quantified stem cell markers OCT4 and SOX2 in hiPSCs and lineage markers like GATA4 (mesoderm), HAND1 (endoderm) and MAP2 (ectoderm) in different embryoid body cells. Our workflow sets a new benchmark in SCP for sensitivity and throughput, with broad applications in basic biology and biomedicine for identification of cell type-specific markers and therapeutic targets.

2024-08-17 | PXD049211 | Pride

Deep single-cell proteomics quantifies low-abundant embryonic transcription factors and lineage markers during induced pluripotent stem cell differentiation

2024-08-17 | PXD054066 | Pride

Deep single-cell proteomics quantifies low-abundant embryonic transcription factors and lineage markers during induced pluripotent stem cell differentiation

2024-08-17 | PXD049181 | Pride

CRISPRi DSP-kd in iAT2

Project description:We performed single cell transcriptomic profiling of induced human pluripotent stem cells (iPSCs)-derived type 2 alveolar epithelial cells (iAT2). iPSCs stably expressed CRISPRi (dCas9-KRAB) under the control of doxycyline. iAT2s were transduced with a lentivirus expressing gRNA targeting the transcriptional start site of DSP. Cells were treated with or without doxycyline to intiate CRISPRi-knockdown. Prior to capture, cells were labelled with hashing antibodies (HTO). Cells were captured for 10x Genomics Single cell capture (V3 protocol), and GEX and HTO libraries were sequenced. HTODemux function was used to demultiplex the samples. Knockdown of DSP caused cells to cluster separately from wild-type cells.

2022-05-26 | GSE189068 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data