Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of neuronal SOX2-ablated murine suprachiasmatic nuclei against wild-type littermate controls

ABSTRACT: RNA-seq of neuronal SOX2-ablated murine suprachiasmatic nuclei against wild-type littermate controls

PROVIDER: PRJEB30100 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2983480_1.fastq.gz	Fastqsanger.gz
	ERR2983480_2.fastq.gz	Fastqsanger.gz
	ERR2983490_1.fastq.gz	Fastqsanger.gz
	ERR2983490_2.fastq.gz	Fastqsanger.gz
	ERR2983500_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 80

Similar Datasets

RNA-seq of neuronal SOX2-ablated murine suprachiasmatic nuclei against wild-type littermate controls

Project description:Mice lacking the stem/progenitor cell transcription factor, Sex determining region Y-box 2 (SOX2), in neurons of the suprachiasmatic nuclei display imprecise, poorly consolidated behavioral rhythms that do not entrain efficiently to environmental light cycles. RNA sequencing revealed that Sox2 deficiency alters the circadian transcriptome of the SCN, reducing the expression of many core clock genes and neuropeptide-receptor systems.

2019-03-18 | E-MTAB-7496 | biostudies-arrayexpress

E-MTAB-7496

Project description:RNA-seq of neuronal SOX2-ablated murine suprachiasmatic nuclei against wild-type littermate controls

2019-08-05 | E-MTAB-7496 | ExpressionAtlas

Genome-wide analysis of Sox2-ablated optic cups compared with wild-type control optic cups at E16.5

2013-05-10 | GSE46796 | GEO

Deciphering responses of the fetal suprachiasmatic nuclei to the rhythmic maternal signals using omics approach

Project description:We report circadian (time series, circadian time 0, 3, 6, 9, 12, 18, 21h) transcriptomic analysis of mouse fetal (embryonic day 17) suprachiasmatic nuclei (SCN) from either sham operarated ad libitum fed mothers (group A) or from SCN-lesioned mothers on resctricted feeding regime. The analysis revealed low amplitude rhythms in the fetal SCN driven by maternal SCN and/or maternal feeding behavior.

2022-04-01 | GSE183172 | GEO

Genome-wide analysis of Sox2-ablated optic cups compared with wild-type control optic cups at E16.5

Project description:To test the hypothesis that ablation of the SOX2 in the developing optic cup leads to expansion of peripheral optic cup signaling pathways, we screened whole-genome changes in gene expression in Sox2-mutant eyes compared with controls. Results confirm that ciliary epithelial (peripheral) identity is gained at the expense of neural retinal identity in Sox2-ablated optic cups. Total RNA was isolated from whole eyes enucleated from six wild-type controls and six Sox2-mutant embryos for a total of twelve eyes per genotype. Each embryo (pair of eyes) was analyzed as a single sample.

2013-05-10 | E-GEOD-46796 | biostudies-arrayexpress

Astrocytes in the mouse suprachiasmatic nuclei respond directly to glucocorticoids feedback

Project description:Astrocytes in the mouse suprachiasmatic nuclei respond directly to glucocorticoids feedback

| PRJNA1005276 | ENA

Circadian VIPergic neurons of the suprachiasmatic nuclei sculpt the sleep-wake cycle

Project description:Circadian VIPergic neurons of the suprachiasmatic nuclei sculpt the sleep-wake cycle

| PRJNA644912 | ENA

Circadian VIPergic neurons of the suprachiasmatic nuclei sculpt the sleep-wake cycle

Project description:Although the mammalian rest-activity cycle is controlled by a "master clock" in the suprachiasmatic nuclei (SCN) of the hypothalamus, it is unclear how firing of individual SCN neurons gates individual features of daily activity. Here, we demonstrate that a specific transcriptomically identified population of mouse VIP+ SCN neurons is active at the "wrong" time of the day -nighttime- when most SCN neurons are silent. Using chemogenetic and optogenetic strategies, we show that these neurons and their cellular clocks are necessary and sufficient to gate and time nighttime sleep, but have no effect upon daytime sleep. We propose mouse nighttime sleep, analogous to the human siesta, is a "hard-wired" property gated by specific neurons of the master clock to favor subsequent alertness prior to dawn (a circadian "wake maintenance zone"). Thus, the SCN is not simply a 24h metronome: specific populations sculpt critical features of the sleep-wake cycle.

2021-01-21 | GSE154038 | GEO

RNA-seq of Mus musculus to investigate the role of RNA editing for the gene expression in the suprachiasmatic nuclei of the hypothalamus

Project description:RNA editing is essential for the formation of functional properties of ionotropic glutamate channels. Previously, we demonstrated the regulation of RNA editing upon manipulation with neural activity in vitro. The hypothalamic suprachiasmatic nuclei (SCN), which serve as a master circadian pacemaker depends on glutamatergic neurotransmission, in particular for signal transmission from the retina, and they exhibit spontaneous 24h rhythmical neural activity. We observed changes in the extent of ionotropic glutamate receptor RNA editing in the SCN during the 24h cycle. Therefore, we aimed to understand the overall role of RNA editing for the mechanism of SCN function and to evaluate the impact of RNA under editing on gene expression.

2023-06-29 | E-MTAB-11408 | biostudies-arrayexpress

Astrocytes in the mouse suprachiasmatic nuclei respond directly to glucocorticoids feedback

Project description:The circadian timing system anticipates daily recurring changes in the environment to synchronize physiology. In mammals, the master pacemaker is the hypothalamic suprachiasmatic nuclei (SCN), which synchronizes “wake” functions by inducing the circadian release of Glucocorticoids (GCs) from the adrenal gland. GCs peak right before the active phase and set the time of peripheral clocks, however, it is still unclear whether the SCN respond to GCs feedback. While GCs influence directly the SCN during the perinatal period, the adult circuit is considered to be resistant to them, suggesting a reduction of GCs-sensitivity along development. To understand this mechanism, we followed the expression of GC receptor (GR) along mouse SCN development with single cell resolution and show that GR is up-regulated in astrocytes as the circuit matures. We provide in vivo and in vitro evidence that the adult SCN stays responsive to circulating GCs through the activation of GR in astrocytes. Astrocytes’ communication is necessary to induce the GC-dependent shift on the SCN clock. Our data provides insight into the development of the SCN and highlight a new role of astrocytes as time-keepers in the adult. This finding might shed light on how the circadian system adapts to jetlag or shift work.

2024-04-10 | GSE240803 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data