Project description:This data is a case study done in the context of developing methods for assessing the taxonomic composition of microbial communities using metaproteomics. For this study with analyzed phototrophic biomats from two Soda Lakes in the Canadian Rocky Mountains using metaproteomics. For protein identification we generated a metagenome from which we predicted and annotated the protein sequences used to analyze the metaproteomes. The database is available in this PRIDE submission. Lake1 refers to Goodenough Lake (GEM, 51°19'47.64"N 121°38'28.90"W) and Lake2 referes to Last Chance Lake (LCM, 51°19'39.3" N 121°37'59.3"W).
Project description:In this study, we used 13C and 15N labeled substrates to assess the carbon (C) and nitrogen (N) assimilation by detecting 13C and 15N in proteins from microorganisms in the phototrophic microbial mats in a soda lake on the Cariboo Plateau, British Columbia, Canada. Metagenomic database used for protein identification and metagenome assembled genome membership had been previousl generated in Zorz et.al. (2019).