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RNA-seq of blood samples from bovines immunized with a multicomponent recombinant vaccine against cattle tick.

ABSTRACT: RNA-seq of blood samples from bovines immunized with a multicomponent recombinant vaccine against cattle tick.

PROVIDER: PRJEB32956 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3365930.fastq.gz	Fastqsanger.gz
	ERR3365940.fastq.gz	Fastqsanger.gz
	ERR3365921.fastq.gz	Fastqsanger.gz
	ERR3365931.fastq.gz	Fastqsanger.gz
	ERR3365941.fastq.gz	Fastqsanger.gz

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RNA-seq of blood samples from bovines immunized with a multicomponent recombinant vaccine against cattle tick.

Project description:Tick infestations by Rhipicephalus microplus, the cattle tick, cause enormous losses to health and animal production. Ticks induce immune response in their hosts; therefore their immunobiological control is feasible. The available anti-tick vaccines display variable efficacy and short-lived, encouraging the search for new protective antigens. The identification of important genes in tick parasitism may indicate protective antigens useful to compose an anti-tick vaccine. We have developed and tested so far four recombinant salivary antigens as a multicomponent anti-tick vaccine in tick-susceptible bovines (Holstein breed). The challenge with R. microplus larvae displayed that tick infestation in vaccinated bovines was significantly reduced. In order to elucidate the molecular mechanisms trigged after immunisation and during infestation, RNA-seq data of peripheral blood from vaccinated and control animals were obtained in different periods of the immunisation trial. A total of 24 mRNA-seq Illumina libraries (single-end, 100 bp) were analysed to identify differential gene expression according to the experimental condition.

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Transcriptional responses in bovine skin during blood feeding of the cattle tick, Rhipicephalus microplus.

Project description:We examined gene expression induced by Rhipicephalus microplus bites on host skin of tick-resistant and tick-susceptible breeds of bovines, Nelore and Holstein respectively, when they underwent a primary infestation.

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Rational identification of novel drug targets and vaccine candidates against the cattle tick, Rhipicephalus microplus

Project description:Control of complex parasites via vaccination remains challenging, with the current combination of vaccines and small drugs remaining the choice of control strategy. The development of new vaccines against complex endo- and ectoparasites remains challenging and more studies are providing evidence that multicomponent vaccines will be needed for the development of protective vaccines against parasites. The development of multicomponent vaccines, require an in-depth understanding of parasite biology which remains insufficient for ticks. With the rapid development and spread of acaricide resistance in ticks, new targets for acaricide development also remains to be identified, along with novel targets that can be exploited for the design of lead compounds. In this study, we analysed the differential gene expression of Rhipicephalus microplus ticks that were fed on cattle vaccinated with a multi-component vaccine (Bm86 and 3 putative Bm86-binding proteins). The data was scrutinised for the identification of vaccine targets, small drug targets and novel pathways that can be evaluated in future studies. Limitations associated with targeting novel proteins for vaccine and/or drug design is also discussed and placed into the context of challenges arising when targeting large protein families and intracellular localised proteins. Lastly, this study provide insight into how Bm86-based vaccines may reduce successful uptake and digestion of the bloodmeal and overall tick fecundity.

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E-MTAB-8022

Project description:RNA-seq of blood samples from bovines immunized with a multicomponent recombinant vaccine against cattle tick.

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Microarray analysis of gene expression in the skin of tick-naïve and tick-infested Holstein-Friesian and Brahman cattle

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Transcription profiling of Bos taurus white blood cells from Brahman and Holstein-Friesian cattle infested with the cattle tick Rhipicephalus microplus

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