Project description:This high-throughput sequencing submission contains H3K27ac CUT&Tag data used to build a source-supported regulatory atlas for foxtail millet, Setaria italica, and a single Setaria viridis validation sample used for representative candidate loci. The data support an H3K27ac-associated regulatory map, sequence-model based candidate cis-regulatory region prediction, and conservative candidate-locus prioritization.
Project description:Transcriptome sequencing of Foxtail millet Setaria italica (Zhang-gu) for different tissues. Four RNA pools were created corresponding to four different tissues: root, leaf, stem, spica (tassel) at developmental stage, then each pool was sequenced.
Project description:Foxtail millet (Setaria italica L. P. Beauv) has been considered as a tractable model crop in recent years due to its short growing cycle, lower repetitive DNA, inbreeding nature, small diploid genome, and outstanding abiotic stress-tolerance characteristics. With modern agriculture often facing various adversities, it’s urgent to dissect the mechanisms of how foxtail millet responds and adapts to drought and stress on the proteomic-level.
Project description:Inflorescence development of foxtail millet (Setaria italica L.) is important for its yield and quality. The molecular basis of foxtail millet inflorescence development is poorly understood. In this study, we analyzed the transcriptome landscape of three developing inflorescence stages in foxtail millet and compared with two-week seedlings. The transcriptome revealed the expression model of genes that may be engagement in inflorescence development, such as kinase, transporter, E3 ubiquitin ligase, reactive oxygen species (ROS) related proteins, MADS-box family, shoot apical meristem pathway related proteins, cell cycle associated proteins, ribosomal proteins and etc. The SiMADS1 knockout line exhibits a phenotype of defective floral organs, resulting in greatly reduced yield. We further analyzed the transcriptomes of simads1 at the green anther and panicle stages. By integrating transcriptomes data with DAP-seq analysis, we identified multiple downstream regulatory genes of SiMADS1. This study provides new genetic resources for further analysis of the molecular mechanism of inflorescence development in foxtail millet.
Project description:To investigate the involvement of arbuscular mycorrhizal symbiosis in the moleular regulation in foxtail millet roots and the effects of genetic variation on AMS-mediated molecular regulation, we isolated total RNA from the roots of 3 different landraces for comprehensive transcriptomic analysis. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different landraces (Hanevalval, TT8, ICE36) after 6-week mock or arbuscular mycorrhizal fungi treatments.
