Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Contrasting genomic signatures of selection driven by insecticide resistance between populations of the major malaria vector Anopheles funestus in Africa

ABSTRACT: Contrasting genomic signatures of selection driven by insecticide resistance between populations of the major malaria vector Anopheles funestus in Africa

PROVIDER: PRJEB35040 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR3610910_1.fastq.gz	Fastqsanger.gz
	ERR3610910_2.fastq.gz	Fastqsanger.gz
	ERR3610911_1.fastq.gz	Fastqsanger.gz
	ERR3610911_2.fastq.gz	Fastqsanger.gz
	ERR3610912_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Transcriptomic profiling of insecticide resistant field Anopheles funestus from Tanzania compared to a fully susceptible laboratory strain

Project description:Comparative gene expression profiling of Anopheles funestus insecticide resistant field strain from an area of a major insecticide treated bednet randomised control trial

2021-06-03 | E-MTAB-10579 | biostudies-arrayexpress

Transcription profiling by array of insecticide resistance in field populations of Anopheles funestus in Benin

Project description:Transcriptome profiling of insecticide resistant field populations of Anopheles funestus from Benin compared to a susceptible lab strain FANG

2013-12-16 | E-MTAB-1578 | biostudies-arrayexpress

Nkya et al 2014 - Insecticide resistance mechanisms associated with different environments in the malaria vector Anopheles gambiae. A case study in Tanzania

Project description:Nine Anopheles gambiae populations were sampled in three areas of Tanzania showing contrasting agriculture activity, urbanization and usage of insecticides for vector control. Insecticide resistance levels were measured in larvae and adults through bioassays with deltamethrin, DDT and bendiocarb. A microarray approach was used for identifying transcription level variations associated to different environments and insecticide resistance. the Ifakara strain originating from central Tanzania and susceptible to all insecticides was used as a reference strain.

2013-10-31 | E-MEXP-3987 | biostudies-arrayexpress

Predictive chemoproteomics and functional validation reveal Coeae6g mediated insecticide cross-resistance in the malaria vector Anopheles gambiae

Project description:This project investigates insecticide resistance mechanisms in Anopheles gambiae using Activity-Based Protein Profiling (ABPP). By targeting serine hydrolases (SHs) with fluorophosphonate (FP) probes and mass spectrometry, we identified COEAE6G as a key enzyme conferring resistance to Pirimiphos-methyl (PM) and cross-resistance to other insecticides. Functional validation, including recombinant protein assays and transgenic mosquito lines, confirmed COEAE6G's role in resistance. This work provides valuable insights into resistance mechanisms, aiding the development of sustainable malaria vector control strategies.

2025-10-17 | PXD060698 | Pride

Pyrethroid resistance mechanisms in Southern African populations of the malaria vector Anopheles funestus

Project description:Transcription profiling of permethrin resistant field mosquito samples of Anopheles funestus from three Southern African populations (Mozambique, Malawi and Zambia) compared to a susceptible lab strain FANG

2014-10-01 | E-MTAB-2216 | biostudies-arrayexpress

Anopheles funestus amidst a South/North shift in the role of key resistance genes across Malawi

Project description:Resistance to pyrethroids, the only insecticide approved for bednets, threatens control of the major malaria vector, Anopheles funestus, in Malawi. To improve the management of such resistance countrywide, it is crucial to understand the dynamics and mechanisms driving resistance in the field. In this study the levels of insecticide resistance were determined across the highly endemic densely populated lake and southern agricultural area. Insecticide resistance to pyrethoids was assessed using standardized WHO bioassay methods and resistant mosquitoes were hybridized to susceptible mosquitoes. This microarray analysis revealed the key role of cytochrome P450 genes such as CYP6P9a, CYP6P9b and CYP6M7. However, a significant shift in the over-expression of these CYP450s was detected across a south/north transect, with CYP6M7 more highly over-transcribed in the two northern collection sites and the tandemly duplicated genes, CYP6P9a and CYP6P9b, more greatly over-transcribed in the south.

2016-02-12 | E-MTAB-3342 | biostudies-arrayexpress

Anopheles funestus strain:South Africa

Project description:Global transcriptomics of male Anopheles funestus

| PRJNA858608 | ENA

Temporal evolution of insecticide resistance and bionomics in Anopheles funestus, a key malaria vector in Uganda.

Project description:Insecticide resistance escalation is decreasing the efficacy of vector control tools. Monitoring vector resistance is paramount in order to understand its evolution and devise effective counter-solutions. In this study, we monitored insecticide resistance patterns, vector population bionomics and genetic variants associated with resistance over 3 years from 2021 to 2023 in Uganda. Anopheles funestus s.s was the predominant species in Mayuge but with evidence of hybridization with other species of the An. funestus group. Sporozoite infection rates were relatively very high with a peak of 20.41% in March 2022. Intense pyrethroid resistance was seen against pyrethroids up to 10-times the diagnostic concentration but partial recovery of susceptibility in PBO synergistic assays. Among bednets, only PBO-based nets (PermaNet 3.0 Top and Olyset Plus) and chlorfenapyr-based net (Interceptor G2) had high mortality rates. Mosquitoes were fully susceptible to chlorfenapyr and organophosphates, moderately resistant to clothianidin and resistant to carbamates. The allele frequency of key P450, CYP9K1, resistance marker was constantly very high but that for CYP6P9A/b were very low. Interestingly, we report the first detection of resistance alleles for Ace1 gene (RS = ~ 13%) and Rdl gene (RS = ~ 21%, RR = ~ 4%) in Uganda. The qRT-PCR revealed that Cytochrome P450s CYP9K1, CYP6P9A, CYP6P9b, CYP6P5 and CYP6M7 were consistently upregulated while a glutathione-S-transferase gene (GSTE2) showed low expression. Our study shows the complexity of insecticide resistance patterns and underlying mechanisms, hence constant and consistent spatial and temporal monitoring is crucial to rapidly detect changing resistance profiles which is key in informing deployment of counter interventions.

| S-EPMC11685729 | biostudies-literature

De novo transcriptome sequencing in Anopheles funestus using Illumina RNA-seq technology

Project description:We report the use of RNA-seq data to assemble transcriptional units of adult Anopheles funestus female mosquitoes. We also analyzed expression levels and protein divergence and discovered SNPs.

2010-12-01 | GSE21977 | GEO

An Africa-wide genomic evolution of insecticide resistance in the malaria vector Anopheles funestus involves selective sweeps, copy number variations, gene conversion and transposons.

Project description:Insecticide resistance in malaria vectors threatens to reverse recent gains in malaria control. Deciphering patterns of gene flow and resistance evolution in malaria vectors is crucial to improving control strategies and preventing malaria resurgence. A genome-wide survey of Anopheles funestus genetic diversity Africa-wide revealed evidences of a major division between southern Africa and elsewhere, associated with different population histories. Three genomic regions exhibited strong signatures of selective sweeps, each spanning major resistance loci (CYP6P9a/b, GSTe2 and CYP9K1). However, a sharp regional contrast was observed between populations correlating with gene flow barriers. Signatures of complex molecular evolution of resistance were detected with evidence of copy number variation, transposon insertion and a gene conversion between CYP6P9a/b paralog genes. Temporal analyses of samples before and after bed net scale up suggest that these genomic changes are driven by this control intervention. Multiple independent selective sweeps at the same locus in different parts of Africa suggests that local evolution of resistance in malaria vectors may be a greater threat than trans-regional spread of resistance haplotypes.

| S-EPMC7297382 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data