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Time-course transcriptome analysis of Sinorhizobium medicae (MD4 strain) in mature nodules of Medicago truncatula grown in split-root under different nitrogen (N) supply

ABSTRACT: Time-course transcriptome analysis of Sinorhizobium medicae (MD4 strain) in mature nodules of Medicago truncatula grown in split-root under different nitrogen (N) supply

PROVIDER: PRJEB35814 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3763000.fastq.gz	Fastqsanger.gz
	ERR3763001.fastq.gz	Fastqsanger.gz
	ERR3763002.fastq.gz	Fastqsanger.gz
	ERR3763003.fastq.gz	Fastqsanger.gz
	ERR3763004.fastq.gz	Fastqsanger.gz

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Time-course transcriptome analysis of Sinorhizobium medicae (MD4 strain) in mature nodules of Medicago truncatula grown in split-root under different nitrogen (N) supply

Project description:The experiment aim is the identification of the transcriptional response of mature nodules of Medicago truncatula inoculated with the strain MD4 of Sinorhizobium medicae in response to different nitrogen (N) supply (N-satiety and N-deficient conditions). We performed a time-course (1 and 3 days treatment) split-root experiment to identify plant systemic responses: the root systems of nodulated symbiotic plants were divided into two compartments, one of them treated and the other kept on control conditions. Nodules were isolated from the non-treated side of the split root and total RNA isolated for RNAseq analysis. To analyse Sinorhizobium medicae MD4 expression, a ribosomal RNA depletion protocol was performed before library preparation.

2020-09-30 | E-MTAB-8598 | biostudies-arrayexpress

Time-course transcriptome analysis of mature nodules in Medicago truncatula grown in split-root under different nitrogen (N) supply

2020-09-30 | E-MTAB-8597 | biostudies-arrayexpress

Response of the mature nodules and denodulated roots of the Medicago truncatula/sinorhizobium medicae md4 symbiotic association to systemic signaling of plant N deficit

Project description:The experiment aims to identify the response of mature nodules and denodulated roots of Medicago truncatula /Sinorhizobium medicae md4 to the systemic signaling of plant N deficit. The root systems of nodulated symbiotic plants were divided into two compartments. The N source of N2 fixing plants was exclusively the air. To reduce the plant nitrogen (N) supply (N-deficient conditions), we flushed one half root system with Ar/O2 (80/20 v/v) while the other half root system was kept on control conditions (Air). To characterize the systemic responses of the transcriptome to plant N deficit , nodules and roots of the non-treated half root system were harvested and total RNA isolated for RNAseq analysis. We performed a time-course (6 hours, 1-, 3- and 5-days treatment).

2021-11-30 | E-MTAB-10496 | biostudies-arrayexpress

Response of the mature nodules of the Medicago truncatula/sinorhizobium medicae md4 symbiotic association to systemic signaling associated to plant water deficit (PEG treatment)

Project description:The experiment aims to identify the response of the mature nodules and denodulated roots of Medicago truncatula /Sinorhizobium medicae md4 to the systemic signaling of plant water deficit. The root systems of nodulated symbiotic plants were divided into two compartments. The N source of N2 fixing plants was exclusively the air. To apply the water stress, we treated half root system with PEG while the other was kept on control conditions. To characterize the systemic responses of the transcriptome to the PEG treatment , nodules and roots of the non-treated half root system were harvested and total RNA isolated for RNAseq analysis. We performed a time-course (6 hours, 1-, 3- and 5-days treatment) .

2021-11-30 | E-MTAB-10497 | biostudies-arrayexpress

Time-course transcriptome analysis in roots of N-limited Medicago truncatula plants grown in split-root inoculated with Sinorhizobium medicae

Project description:The experiment aims to analyze the effect of inoculation with Sinorhizobium medicae MD4 of on the transcriptome of the Medicago truncatula roots of N-limited plants. We divided the roots systems into two parts; one of them was supplied with nutrient solution with mineral N (0.5 mM for N-limited plants) whereas the non-N treated part was kept in nutrient solution without mineral N. All roots were inoculated 48h after initiation of the N treatments. We collected the roots the non-N treated side of the split roots system and we isolated total RNA for RNAseq analysis. Root transcriptomes were analyze before inoculation, at 1 and 2 days post-inoculation.

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Genome of Ensifer medicae MD4

Project description:Genome of Ensifer medicae MD4 an efficient symbiont of Medicago truncatula

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Effect of contrasted N regimes on the root transcriptome of Medicago truncatula plants cultivated in split root systems and inoculated for 2, 4 and 7 days with Sinorhizobium medicae

Project description:The experiment aims to analyze the effect of systemic signaling of plant N demand on the transcriptome of Medicago truncatula roots inoculated with Sinorhizobium medicae MD4 at 2, 4, and 7 days post-inoculation (dpi). We divided the roots systems into two parts; one of them was supplied with nutrient solution with mineral N (10 mM NH4NO3 for N-satisfied plants, 0.5 mM for N-limited plants) whereas the non-N treated part was kept in nutrient solution without mineral N. All roots were inoculated 48h after initiation of the N treatments. To characterize specifically the impact of systemic signaling of N demand, we collected the roots the non-N treated sides of the split roots systems and we isolated total RNA for RNAseq analysis. Root transcriptomes of N-limited and N-satisfied plants were compared at 2, 4 and 7 dpi.

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Time-course transcriptome analysis of mature nodules in Medicago truncatula grown in split-root under different nitrogen (N) supply

Project description:Time-course transcriptome analysis of mature nodules in Medicago truncatula grown in split-root under different nitrogen (N) supply

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Response of the mature nodules of the Medicago truncatula/sinorhizobium medicae md4 symbiotic association to systemic signaling of plant N deficit

Project description:Response of the mature nodules of the Medicago truncatula/sinorhizobium medicae md4 symbiotic association to systemic signaling of plant N deficit

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Response of the mature nodules of the Medicago truncatula/sinorhizobium medicae md4 symbiotic association to systemic signaling associated to plant water deficit (PEG treatment)

Project description:Response of the mature nodules of the Medicago truncatula/sinorhizobium medicae md4 symbiotic association to systemic signaling associated to plant water deficit (PEG treatment)

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